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Entry prohibited for AIDS viruses: Peptide triazole inhibitors disrupt cell-free HIV-1

July 8, 2011



(PhysOrg.com) -- The initial entry of HIV-1 into host cells remains a compelling yet elusive target for the development of agents to prevent infection, a critical need in the fight against the global AIDS epidemic.

A collaborative research effort led by Irwin Chaiken at Drexel University and Drexel University College of Medicine (Philadelphia, PA, USA) has demonstrated that modified peptide triazole inhibitors which specifically target the HIV-1 envelope (Env) protein gp120 can physically disrupt virus particles in the absence of host cells, and the results are reported in ChemMedChem.

The Drexel team found that under conditions similar to those at which a newly designed peptide triazole (KR13) inhibits infection of host cells by an HIV-1 pseudovirus, it also causes virus rupture and release of an internal HIV-1 protein called gag p24 when incubated with virus alone.

Both inhibition of cell infection and p24 release are enhanced substantially by the multivalent display of KR13 on gold nanoparticles.

The novel antagonist design and reported characterization data could lead to the creation of a virucide to suppress initial HIV-1 infection, viremia in infected individuals, and the spread of infection between individuals. Such agents could be used for HIV-1 microbicides and therapeutics.

These results also suggest that ligand-specific pathogen rupture may be possible for other viruses that contain metastable prefusion surface protein complexes, such as influenza, Ebola, and Dengue.

More information: Irwin Chaiken, Cell-Free HIV-1 Virucidal Action by Modified Peptide Triazole Inhibitors of Env gp120, ChemMedChem 2011, 6, No. 8, http://dx.doi.org/ … dc.201100177

http://www.physorg.com/news/2011-07-entry-prohibited-aids-viruses-peptide.html

Virucides:
The goal of this project is to test the hypothesis that a mature HIV particle is metastable against membrane poration due to a combination of virus spike protein metastability and high internal Laplace pressure. We seek molecular constructions that will bind simultaneously to virus spike and membrane, and disrupt membrane integrity sufficiently to cause irreversible leakage of viral contents, rendering the virus noninfectious.
http://www.drexelmed.edu/Home/AboutTheCollege/DepartmentsCentersandInstitutes/BasicScienceDepts/BiochemistryandMolecularBiology/Faculty/ChaikenResearchGroup/Research.aspx