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Re: Paulness post# 18749

Wednesday, 05/18/2005 8:20:45 PM

Wednesday, May 18, 2005 8:20:45 PM

Post# of 64738
Very Very Nice

Cell-free amplification of C-DNA has many important benefits beginning with the size and composition of the plasmid. Under this system, there is no need for bacterial replication genes or selection markers such as antibiotic resistant genes. In most cases, this will reduce the size and weight of the therapeutic C-DNA by at least 3,000 base pairs or a molecular weight of approximately 2,000 kilo Daltons. The total absence of bacteria and growth media assures that there is no need to employ mechanical or chemical purification methods to extract cell or animal proteins, RNA, genomic DNA and backbone molecules. This feature allows the designer more control of coding for non-specific and specific immune responses.
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