American Energy Partners Inc (AEPT)

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Alexander's Son, Greg Fridman, MUST READ!

The direct plasma treatment implies that living tissue itself is used as one of the electrodes and directly participates in the active plasma discharge processes. Thus Figure 1 illustrates direct plasma treatment(sterilization) of skin of a live mouse. Dielectric Barrier Discharge (DBD) plasma is generated in this case between the quartz-surface covered high-voltage electrode and the mouse which serves as a second electrode.

The FE-DBD plasma treatment was shown to initiate apoptosis in Melanoma cancer cell lines – a threshold at which plasma treatment does not cause immediate necrosis but initiates complex cascade of biochemical processes leading to cell death many hours and even days following the treatment [27].
Melanoma cells, treated by plasma at doses significantly below those required for cell destruction, survive the plasma treatment but develop apoptosis many hours post treatment and die (disintegrate) by themselves gracefully. This could potentially be an intriguing approach for cancer treatment, especially if by manipulation of plasma parameters the treatment could be made selective to cancerous cells over healthy cells, as was demonstrated before for bacteria vs. healthy cells [19, 20].

A radio frequency plasma source, a plasma needle, was recently developed by E. Stoffels et al. [18, 130-
132]. Plasma needle is a flexible hand-held device (Figure 30) consisting of a 0.3 mm diameter needle,
0.8 mm diameter Perspex tube, 10 cm in length. The plasma is generated at the end of the needle at the
applied frequency of 13.56 MHz. This device was successfully demonstrated in treatment of various cell
lines and inactivation of bacteria. Though the final goal of this plasma treatment is in treatment of dental
cavities, localized and precise inactivation of cancerous tissues, and in other medical applications, at the
moment deeper understanding of biological mechanisms of plasma-cell interaction mechanisms is being
pursued [133]. Dr. Stoffels and her colleagues have thus-far worked with the following eukaryotic cells
and bacteria [18]:

Figure 33. Facial appearance before (A) and 3 months after (B) plasma skin regeneration, with improvement in pigmentation and skin texture. Investigator-rated improvement on the 9-point facial rhytid scale changed from 7 (before regeneration) to 6 (after regeneration); patient-rated improvement in overall skin rejuvenation was 90% [10].

http://www.gregfridman.com/publications/documents/PPP_review_Plasma_Medicine.pdf