Fate Therapeutics Inc (FATE)

[jondoeuk]

Has any bio co-cultured their CARNK with TAM and cancer cell lines?

No, but one of the co-founders of Shoreline showed that while macrophages alone did not kill AML blasts, the addition of NKs (both without a CAR) significantly improved killing of AML blasts by half. The addition of an anti-CD47 mAb further increased killing by 23%.

Another (academic) group has created iPSC-derived CAR-macrophages, CAR-NKs, CAR-eosinophils, and CAR-neutrophils. Some of the preclinical data shows that both CAR-M and CAR-NK suppressed cancer cell survival more efficiently than either alone, even at higher doses of these cells. Also that the phagocytosis activity of CAR-M was increased in the presence of CAR-NK. In addition, CAR-eosinophils improved CAR-NK activity, and CAR-M with CAR-eosinophils enhance the activity of CAR-NK.

Anyone testing CARNK + ATEZO?

Not that I'm aware. One group showed that combination of allo NKs with pembrolizumab was well-tolerated and improved PFS and OS in patients (with NSCLC) compared with pembrolizumab alone https://www.jci.org/articles/view/132712

Also, a PhI testing allo tumour-reactive PD-L1+ NKs engineered to express soluble IL-15 in patients with NSCLC refractory to PD-1/PD-L1 inhibitors is ongoing.