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Tuesday, November 29, 2022 11:30:05 AM
This is great. It's different from the other expertise that KBLB already employs.
This position focuses on electroporation. I've been following this field for a couple years now and it is very exciting. A few gene editing companies I follow use this technique in order to get AAV's past the Blood Brain Barrier in order to get the genetic payloads into hard to reach areas of the brain.
Electroporation is basically a technique where you apply an electric field to a membrane and it vibrates (for simplicity sake) the wall of the membrane and allows your target to pass through the barrier. It's a pretty fast growing field.
I had never heard of this technique being used in insect eggs but I suppose it makes a lot of sense. Theoretically, you could use electroporation to insert plasmids across an egg shell just like you would to any other cell membrane (considering all eggs are just a single cell), and you could do it on a large scale very quickly and efficiently. This would save a ton of time compared to the normal technique of microinjecting the plasmid into the egg and literally supergluing the hole (yes, that is the how the field currently does it).
This technique will allow for large amounts of eggs to be edited in very short order. Kraig labs will always be improving their genetic constructs and creating custom inserts for customers so this could help them create custom fibers very quickly.
Great find and thanks for the share
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