BioAmber Inc fka BIOAQ

[whitegold3]

they are using Bioamber's technology
The overseas BioAmber method and Roquette (DSM/Roquette) method are used to synthesize succinic acid by synthetic biology, and the domestic Shandong Landian technology is relatively mature. The natural metabolic pathway of succinate goes through the process of glycolysis-tricarboxylic acid cycle. In the process of strain modification, the main purpose is to inhibit the conversion of pyruvate to non-succinate pathway during the metabolic process, and to construct the corresponding energy metabolism regulation process .

In the transformation path of BioAmber, the chassis cells selected I. orientalis, firstly knocked down the conversion pathway of pyruvate to ethanol, and at the same time, through the modification of enzyme structure/introduction of exogenous enzymes, the conversion from pyruvate to ethanol in the TCA reduction pathway was strengthened. Pathway from oxaloacetate to malate to fumarate and finally to succinate. At the same time, it inhibits the conversion of L-glutamine to UTP in terms of energy metabolism regulation to ensure the supply of NADH. In terms of yield, BioAmber was 0.45 wt%; concentration was 48.2 g/L; yield was 0.97 g/L·h. https://zshq.zuiyouliao.com/zixun/detail-22d188ca3cd64c74a29622419743fb0a.html