Co-Diagnostics Inc (CODX)

[Raffis]

https://codiagnostics.com/wp-content/uploads/2018/11/Journal_of_Molecular_Diagnostics_Article.pdf“The increasing need to multiplex nucleic acid reactions presses test designers to the limits of amplifi- cation specificity in PCR. Although more than a dozen hot starts have been developed for PCR to reduce primer-dimer formation, none can stop the propagation of primer-dimers once formed. Even a small number of primer-dimers can result in false-negatives and/or false-positives. Herein, we demonstrate a new class of primer technology that greatly reduces primer-dimer propagation, showing successful amplification of 60 template copies with no signal dampening in a background of 150,000,000 primer- dimers. In contrast, normal primers, with or without a hot start, experienced signal dampening with as few as 60 primer-dimers and false-negatives with only 600 primer-dimers. This represents more than a 2.5 millionefold improvement in reduction of nonspecific amplification. We also show how a probe can be incorporated into the cooperative primer, with 2.5 times more signal than conventional fluorescent probes. (J Mol Diagn 2013, -: 1e11; http://dx.doi.org/10.1016/j.jmoldx.2013.10.004).”