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Re: nonameface13 post# 52027

Sunday, 01/24/2021 11:14:15 AM

Sunday, January 24, 2021 11:14:15 AM

Post# of 54983
Do different cigarettes have different nicotine levels?

Yes they do.

BTI said the lower leaves of tobacco plants have much less

nicotine than the upper leaves and with blending they can achieve

any nicotine level desired.

Do facts matter?

Why did BTI pass on XXII patents?

Anyone here ever hear of "grafting"?
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Different products have different levels of nicotine. The average amount of nicotine in a single cigarette is around 10 to 12 mg. This can vary widely from one brand to the next. Besides nicotine, cigarettes contain hundreds of other substances, many of which can be harmful to your health.Nov 18, 2019
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Grafting:

Reducing the nicotine content of tobacco by grafting with eggplant
Mengjuan Ren, Mengyue Zhang, Huijuan Yang & Hongzhi Shi
BMC Plant Biology volume 20, Article number: 285 (2020) Cite this article

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Abstract
Background
Nicotine is a stimulant and potent parasympathomimetic alkaloid that accounts for 96–98% of alkaloid content. A reduction in the amount of nicotine in cigarettes to achieve a non-addictive level is necessary. We investigated whether replacing tobacco root with eggplant by grafting can restrict nicotine biosynthesis and produce tobacco leaves with ultra-low nicotine content, and analyzed the gene expression differences induced by eggplant grafting.

Results
The nicotine levels of grafted tobacco leaves decreased dramatically. The contents of nornicotine, anabasine, NNN, NNK, NAT, total TSNAs and the nicotine of mainstream cigarette smoke decreased, and the contents of amino acids and the precursors of alkaloids increased in grafted tobacco. Eggplant grafting resulted in the differential expression of 440 genes. LOC107774053 had higher degrees in two PPI networks, which were regulated by LOC107802531 and LOC107828746 in the TF-target network.

Conclusions
Replacing tobacco root with eggplant by grafting can restrict nicotine biosynthesis and produce tobacco leaves with ultra-low or zero nicotine content. The differential expression of LOC107774053 may be associated with eggplant grafting.

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