PATENTS ASSIGNED TO SPIBER INC.- PAGE 1
Expression Cassette
Publication number: 20200308552
Abstract: Disclosed is an expression cassette comprising, in a 5? to 3? direction of a sense strand, a promoter, and a first nucleic acid, a terminator and a second nucleic acid operably linked to the promoter, wherein the first nucleic acid and the second nucleic acid each contain at least one gene.
Type: Application
Filed: September 28, 2018
Publication date: October 1, 2020
Applicant: Spiber Inc.
Inventor: Masahiro Murakami
Method for Producing Double-Stranded DNA Fragments
Publication number: 20200248231
Abstract: The present invention relates to a method for producing a double-stranded DNA fragment having a desired nucleotide sequence through dual asymmetric PCR (DA-PCR). The method comprises: (1) providing a plurality of oligonucleotides (sense oligonucleotides) each corresponding to a part of a sense strand of the double-stranded DNA fragment and a plurality of oligonucleotides (antisense oligonucleotides) each corresponding to a part of an antisense strand of the double-stranded DNA fragment and mixing together the oligonucleotides with equal concentrations, DNA polymerase, and dNTP to prepare a reaction mixture solution; (2) performing PCR by using the reaction mixture solution from step (1); (3) adding a primer set capable of amplifying the double-stranded DNA fragment of full length to the reaction mixture solution from step (2); and (4) performing PCR by using the reaction mixture solution from step (3).
Type: Application
Filed: August 28, 2018
Publication date: August 6, 2020
Applicant: Spiber Inc.
Inventors: Yusuke Ito, Chikako Sato, Nozomu Yachie
Modified Fibroin
Publication number: 20200207817
Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences.
Type: Application
Filed: July 25, 2018
Publication date: July 2, 2020
Applicants: Spiber Inc., Riken
Inventors: Keisuke Morita, Yunosuke Abe, Takehisa Maekawa, Koichi Kotaka, Junichi Sugahara, Keiji Numata
Polypeptide Solution, Production Method for Polypeptide Fiber, and Artificial Polypeptide
Publication number: 20200165311
Abstract: The present invention relates to a polypeptide solution in which polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.
Type: Application
Filed: May 9, 2018
Publication date: May 28, 2020
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Ayumi Abe, Keisuke Morita, Kenji Kurachi, Yunosuke Abe
Composite Material and Composite Material Manufacturing Method
Publication number: 20200130335
Abstract: A composite material 100 is obtained by laminating and tightly adhering a fabric 60 containing fibers made of spider silk fibroin and a synthetic resin film 50 having a tensile elongation equal to or greater than that of the fabric 60.
Type: Application
Filed: July 3, 2018
Publication date: April 30, 2020
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Kazuhiro Uchida, Masahiro Asakawa, Hiroyuki Mori, Wataru Ishida, Shinya Ueda
Protein Molded Article And Method For Producing Same, Protein Solution, And Protein Molded Article Plasticizer
Publication number: 20200094446
Abstract: The present invention relates to a protein molded article including a protein, in which an ionic liquid is included inside the protein molded article. The present invention also relates to a production method of the protein molded article, including a step of adding the ionic liquid to a molding material of the protein molded article. Further, the present invention relates to a protein solution including a protein, an ionic liquid, and a solvent. Further, the present invention also relates to a protein molded article plasticizer including an ionic liquid.
Type: Application
Filed: May 31, 2018
Publication date: March 26, 2020
Applicants: Institute of National Colleges of Technology, Japan, Spiber Inc.
Inventors: Takaya SATO, Takashi MORINAGA, Ryo SATOH, Kentaro TAKAHASHI, Takehiro SATO
Highly Contracted Synthetic Fibroin Fiber, Production Method Therefor, and Method for Contracting Synthetic Fibroin Fiber
Publication number: 20200031887
Abstract: The present invention relates to a highly contracted artificial fibroin fiber including a modified fibroin, in which a contraction percentage defined by the following equation exceeds 7%.
Type: Application
Filed: March 5, 2018
Publication date: January 30, 2020
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Junichi Sugahara, Keisuke Morita, Hiroyuki Nakamura
Method and Device for Manufacturing Protein Fiber
Publication number: 20200031886
Abstract: The present invention relates to a method for manufacturing a protein fiber, including an extension and contraction step of contracting or extending a protein raw fiber containing a protein by bringing the protein raw fiber into contact with a liquid or vapor; and a drying step of drying the protein raw fiber that has undergone the extension and contraction step while adjusting a length of the protein raw fiber to an arbitrary length.
Type: Application
Filed: March 5, 2018
Publication date: January 30, 2020
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Takashi Morinaga, Takehisa Maekawa
Microbe Proliferation Inhibitor, Method for Inhibiting Proliferation of Microbes, UV Resistance-Improving Agent, Method for Improving UV Resistance, Artificial Protein Formation Body and method for Manufacturing Same, Artificial Protein Solution, and Coloring Agent
Publication number: 20200000091
Abstract: One aspect of the present invention is a microbial growth inhibitor comprising xanthurenic acid.
Type: Application
Filed: February 28, 2018
Publication date: January 2, 2020
Applicants: Keio University, Spiber Inc.
Inventors: Kazuharu Arakawa, Masayuki Fujiwara, Nobuaki Kono, Yoshinori Ohta
Protein Composition, Method for Producing Same and method for Improving Heat Stability
Publication number: 20190352349
Abstract: The present invention relates to a protein composition formed of dry matter of a dispersion in which a protein is dispersed in a stabilizer solution, in which the stabilizer includes at least one type selected from the group consisting of a catechol-based stabilizer, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 2,6-di-tert-butyl-p-cresol, 2-tert-butyl-4-methoxyphenol, and 6,6?-di-tert-butyl-4,4?-butylidenedi-m-cresol.
Type: Application
Filed: November 29, 2017
Publication date: November 21, 2019
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Toshiaki Taniike, Anh Thi Ngoc Dao, Koyuru Nakayama, Junichi Shimokata, Kengo Takeuchi, Abhijit Ravikiran Muley, Sunita Darbe
Method for Recovering Protein
Publication number: 20190352330
Abstract: The present invention relates to a method for recovering protein, which recovers a target protein from a mixture containing the target protein and a material different from the target protein, the method including a dissolution step of dissolving either the target protein or the material by applying pressure while heating to a solution for dissolution containing the mixture and a polar solvent, and a separation step of separating an obtained solution.
Type: Application
Filed: December 25, 2017
Publication date: November 21, 2019
Applicant: Spiber Inc.
Inventors: Kazuhide Sekiyama, Akihiko Ozeki, Ryoji Okada, Koichi Kotaka
Modified Fibroin
Publication number: 20190233481
Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence locally containing a region with locally high hydropathy index equivalent to an amino acid sequence in which one or a plurality of amino acid residues in REP are substituted with amino acid residues with a high hydropathy index and/or one or a plurality of amino acid residues with a high hydropathy index are inserted into REP, as compared to naturally occurring fibroin. In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300, A plurality of (A)n motifs and the like may be the same amino acid sequence or different amino acid sequences.
Type: Application
Filed: June 23, 2017
Publication date: August 1, 2019
Applicants: Spiber Inc., KOJIMA INDUSTRIES CORPORATION
Inventors: Keisuke MORITA, Koichi KOTAKA
Method for Purifying Recombinant Protein
Publication number: 20190225646
Abstract: Disclosed is a method for purifying a recombinant protein of interest from a recombinant cell expressing intracellularly the recombinant protein as an insoluble matter, comprising treating the recombinant cell under conditions where a protein derived from a host cell dissolves in a first aprotic polar solvent to which an inorganic salt is added or not but the recombinant protein does not dissolve, removing a first soluble fraction, and then treating the resulting first insoluble fraction under conditions where the recombinant protein dissolves in a second aprotic polar solvent to which an inorganic salt is added.
Type: Application
Filed: October 3, 2017
Publication date: July 25, 2019
Applicant: Spiber Inc.
Inventor: Toshimasa Homma
Method for Producing Recombinant Protein
Publication number: 20190194710
Abstract: The invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused.
Type: Application
Filed: August 2, 2017
Publication date: June 27, 2019
Applicant: Spiber Inc.
Inventors: Kenji Kurachi, Mihoko Kinoshita
Molded Article and Method for Producing Molded Article
Publication number: 20190194403
Abstract: The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.
Type: Application
Filed: September 1, 2017
Publication date: June 27, 2019
Applicants: Spiber Inc., Tekunohama Co., Ltd.
Inventors: Shinji Hirai, Yukimasa Ubukata, Ayumi Abe, Koichi Kotaka, Yuki Nakayama, Junichi Noba
Spider silk protein film, and method for producing same
Patent number: 10329332
Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.
Type: Grant
Filed: December 17, 2013
Date of Patent: June 25, 2019
Assignee: SPIBER INC.
Inventors: Kaori Sekiyama, Mizuki Ishikawa, Shinya Murata
COMPOSITE MOLDING COMPOSITION INCLUDING FIBROIN-LIKE PROTEIN, AND METHOD FOR PRODUCING COMPOSITE MOLDING COMPOSITION
Publication number: 20190186050
Abstract: Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a ?-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein.
Type: Application
Filed: July 24, 2017
Publication date: June 20, 2019
Applicants: RIKEN, SPIBER INC.
Inventors: Keiji NUMATA, Kousuke TSUCHIYA, Junichi SUGAHARA, Takaoki ISHII, Hongfang CHI
Production Method for Insoluble Recombinant Protein Aggregate
Publication number: 20190177363
Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.
Type: Application
Filed: August 10, 2017
Publication date: June 13, 2019
Applicant: Spiber Inc.
Inventors: Toshiaki Osawa, Yuya Sato, Keisuke Morita
Modified Fibroin
Publication number: 20190135880
Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of (A)n motif equivalent to an amino acid sequence in which, at least, one or a plurality of the (A)n motifs is deleted, as compared to naturally occurring fibroin. [In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.
Type: Application
Filed: April 28, 2017
Publication date: May 9, 2019
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Keisuke Morita, Hiroyuki Nakamura
Modified Fibroin
Publication number: 20190135881
Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin: [In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.
Type: Application
Filed: April 28, 2017
Publication date: May 9, 2019
Applicants: Spiber Inc., Kojima Industries Corporation
Inventors: Keisuke Morita, Junichi Sugahara
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