Arrayit Corporation (ARYC)

[Alyssa]

Multiplexed Detection and Quantification of Human Antibody Response to COVID-19 Infection Using a Plasmon Enhanced Biosensor Platform

EXCERPT:

For use in the COVID-19 detection assay, GC-FP chips were printed with an array of approximately 400 µm diameter spots of target and control antigens/proteins using an ArrayIt SpotBot II microarray printer. All printed proteins/antigens were first diluted to 500 µg/µl in phosphate buffered saline (PBS) and then further diluted 1:1 just prior to printing with GBL protein array printing buffer (Grace Bio-Labs) All proteins/antigens used for printing were kept at -20 °C at 500 µg/µl for long-term storage. Target antigen spots were printed in triplicate, while negative and positive controls were printed in duplicate or triplicate on each GC-FP biosensor chip. After printing, GC-FP biosensor chips were allowed to dry in ambient conditions for a minimum of 30 min, then placed in a desiccant-filled box for long term storage.

https://www.medrxiv.org/content/10.1101/2020.09.02.20187070v2.full.pdf