Decision Diagnostics Corp (DECN)

[Raffis]

“A novel competitive electrochemical immunosensor for MERS-CoV which was carried out on a DEP array electrodes nanostructured with gold nanoparticles was developed. The detection relied on the indirect competition between the free virus in the sample and immobilized MERS-CoV protein for fixed concentration of added antibody to the sample. The sensor response was detected by monitoring the change in the peak current of the SWV signal upon adding different concentrations of the MERS-CoV antigen. This immunosensor showed low detection limit, high degree of selectivity against other non-specific proteins (Flu A and Flu B) and acceptable stability. The sensitivity of MERS-CoV immunosensor is attributed to the utilization of AuNP modified carbon array electrodes which lead to increased electron transfer efficiency and electrode surface area. Furthermore, the use of disposable DEP array electrode reduces the cost of the assay and enabled the multiplexed detection of MERS-CoV and HCoV simultaneously. Finally, the competitive immunosensor was successfully used to detect MERS-CoV and HCoV proteins in spiked nasal samples showing good recovery percentages.”
https://link.springer.com/article/10.1007/s00604-019-3345-5