Wednesday, March 25, 2020 3:36:44 PM
Below is Google translation of an article by a Korean scientist on one of major Korean news media on 3-24-2020.
I think it shouldn't be too difficult for Decision Diagnostic Co to develop a testing kit for new virus.
We see many companies jumped in doing this business lately and sucessful. It's not like the company is developing a new drug.
And if FDA does not accept GenViro, the company can sell the products in other countries.
And of course you can not trust a small OTC company 100%. That is the dilemma here.
---------------------------------------------------------------------
Testing Kit development is not difficult.The most important thing is to find out the structure of a specific gene or genome of a corona virus. Corona virus has few genes, so experts can understand the overall nucleotide structure within a few days. Structures have already been identified in several places. You can make a kit by referring to it.
The order is like this. First of all, the target gene to be amplified is determined among the revealed genes of corona. Next, around 20 short oligonucleotides that can be attached to the end of the gene are synthesized. This is the key to kit development. This is called a primer and is necessary for amplification of the gene. Primer is necessary to synthesize a gene (see Figure 1 below). It is also easy to make; synthesizing can be done by a 3rd party company specialized for it.
There are several types of kits because the target gene to be amplified varies depending on the development company. Naturally, the resulting primers are also different. Only this difference. This difference may have some effect on accuracy, but it is not very different. Kit preparation is as simple as purchasing an enzyme that synthesizes genes and mixing primers, substrates (nucleic acid groups, nucleotides, A, G, C, T), and buffers. Of course, you need to get the product approval from the competent department. Here, the substrate is the building material for genetic synthesis (?) And is used by the purchaser. Buffer is a simple reagent that keeps the pH of the enzyme reaction constant during amplification. Five domestic small and medium-sized enterprises are producing in large quantities. Even during this period when demand explodes, there is still supply. You can purchase this kit in the field, add a sample, put it in a machine (thermal cycler) and follow the manual.
Samples are collected by scraping them with a cotton swab deep into the nostrils or throat. Theoretically, you only need one or two viruses. If it is collected incorrectly, it may come out negatively. Sneeze splash or cough sputum is better than these samples. As a sample of PCR, a sample (eg, RNA) extracted by melting a virus with an enzyme in a test tube is used as a sample. A very small amount of genome is amplified and amplified the target (target) gene by PCR.
[Source: JoongAng Ilbo] [The Longer] The World's Surprised Corona Test Speed, How to ...
FEATURED Nightfood Signs Letter of Intent to Acquire Los Angeles Cooking School, Integrating Automation and Robotics with World-Class Culinary Training • Oct 1, 2024 8:30 AM
FEATURED ZenaTech, Inc. (NASDAQ: ZENA) To Commence Trading Today • Oct 1, 2024 7:00 AM
Integrated Ventures, Inc Reports Total 2024 Revenues Of $5,863,935 vs $3,862,849 for 2023. • INTV • Oct 1, 2024 9:00 AM
Element79 Gold Corp secures loi for launching tailings reprocessing business in Arequipa, Peru • ELMGF • Oct 1, 2024 6:38 AM
BARRON'S COVE to Premier at the Hamptons International Film Festival • APHP • Sep 30, 2024 2:56 PM
Lingerie Fighting Championships Signs Broadcast Deal With Maybacks Global Entertainment • BOTY • Sep 26, 2024 9:00 AM