Avid Bioservices manufactures for Halozyme re: PEGPH20 and ENHANZE and some more research out on PEGPH20 which further should help lock up Avid manufacturing contracts
The question is as PS Targeting IP assets remodels the TME ( Tumor Microenvironment) and when it comes to remodeling the TME the IP held by Peregrine ...now Oncologie via a John Springs Stafford act, should raise MANY questions by all
I like how CDMO is on the rise but is it possible that Avid being guaranteed manufacturing rights in exchange for something else ...
Looks like Johnson / Johnson just poached Halozyme employee Sheryl Apostol Garrovillo who is well aware the TME
Johnson and Johnson just may like the way Anti-PS changes the TME and and I imagine JnJ would not be pleased if Oncologie was blocking them from IO combos with Bavi etc ...
Merck doesn't seem to have any problems using Keytruda with Bavi
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Remodeling the Tumor Microenvironment Sensitizes Breast Tumors to Anti- Programmed Death-Ligand 1 Immunotherapy
Renee Clift, Jennifer Souratha, Sheryl A. Garrovillo, Susan Zimmerman and Barbara Blouw
Abstract
Immunotherapies targeting immune checkpoint inhibitors have changed the landscape of cancer treatment, however, many patients are resistant or refractory to immunotherapy. The sensitivity of tumor cells to immunotherapy may be influenced by hyaluronan (HA) accumulation in the tumor microenvironment (TME). Enzymatic degradation of HA by pegvorhyaluronidase alfa (PEGPH20; PVHA) remodels the TME. This leads to reduced tumor interstitial pressure and decompressed tumor blood vessels which are both associated with increased exposure of tumor cells to chemotherapy drugs. Here, we demonstrate pegvorhyaluronidase alfa increased the uptake of anti-programmed death-ligand 1 (PD-L1) antibody in HA-accumulating animal models of breast cancer. The increased levels of anti-PD-L1 antibody were associated with increased accumulation of T-cells and natural killer cells, and decreased myeloid-derived suppressor cells. PD-L1 blockade significantly inhibited tumor growth when combined with pegvorhyaluronidase alfa, but not alone. Our results suggest that pegvorhyaluronidase alfa can make HA-accumulating tumors sensitive to anti-PD-L1 immunotherapy.
Received September 28, 2018.
Revision received January 22, 2019.
Accepted June 24, 2019.
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Experience
Senior Associate Scientist
The Janssen Pharmaceutical Companies of Johnson & Johnson
May 2019 â?? Present 3 months
3210 Merryfield Row, San Diego, CA 92121
Halozyme Therapeutics, Inc.
5 years
Senior Histopathology Technician II
January 2018 â?? April 2019 1 year 4 months
11388 Sorrento Valley road, San Diego, CA 92121
...
...
Responsible for hands-on implementation of the histology laboratory providing high quality histology services
Prepare tissues for histological evaluation including use of immunohistochemistry staining techniques to support preclinical development and collaborations
Representative of histology group for Protocol Reviews
Team lab. Captain for Research Operational Team
Support vivarium necropsy on large studies and train other associates on optimal tissue embedding techniques for optimal histology read out
Develop, optimize antibody, cross-reactivity studies and validate different immunohistochemistry techniques and staining methods for preclinical and clinical studies
Prepare tissues for IHC: grossing, fixing, processing, embedding, trimming and sectioning
Perform histochemistry, immunohistochemistry, immunofluorescence, FISH/ISH based assays
Perform paraffin and cryostat sectioning
Validate and troubleshoot technical problems in assay development
Maintaining inventory of paraffin blocks and slides
Archiving blocks and stain/unstained slides
Stocking, inventory and order lab supplies
Manage histo lab equipment and microscopes for routine PM services
Helps in developing multiplex staining in Leica Bond and Ventana Machine
Senior Histopathology Technician 1
May 2014 â?? January 2018 3 years 9 months
11388 Sorrento Valley Blvd
Responsible for hands-on implementation of the histology laboratory providing high quality histo services.
Prepare tissues for histological evaluation including use of immunohistochemistry staining techniques to support preclinical development and collaborations.
Representative of histology group for Protocol Reviews.
Team lab. Captain for Research Operational Team.
Support vivarium necropsy on large studies and train other associates on optimal tissue embedding techniques for optimal histology read out.
Prepare tissues for IHC: grossing, fixing, processing, embedding, trimming and sectioning.
Perform paraffin and cryostat sectioning.
Perform H&E, special staining and IHC staining as needed.
Developing a new IHC assays.
Quality control on finished slide preparations.
Maintaining inventory of paraffin blocks and slides.
Archiving blocks and stain/unstained slides.
Stocking, inventory and order lab supplies,
Manage histo lab equipment and microscopes for routine PM services.
Load slides in Leica Aperio scanner.
Doing IHC staining in Ventana machine, Leica Bond Max and Leica Bond III.
