Kraig Biocraft Laboratories Inc (KBLB)

[TRUISM]

ABSTRACT

It is the transgenic silkworm which becomes with gene manipulation in order to hold the chimera spider silk protein gene and be revealed, and the transgenic silkworm in which the chimera spider silk protein gene encodes the spider silk elasticity motif order and/or the chimera spider silk protein having the specific spider silk domain about the spider silk intensity motif order is disclosed.

Moreover, the silk fiber is provided to the intensity improved in the nature fistula in comparison with the silk fiber and the fistula which shows the spring characteristic and is improved (extension). It the method for using the piggyback vector system and helper plasmid as the improved method for manufacturing the chimera silk fiber for using the disclosed transgenic silkworm for the present application is moreover provided. The gene expression cassette is provided and it is used to manufacture various composition spider silk encryption sequence (the spider 2, spider 4, spider 6, spider 8).

The piggyback vector system is used when in order that the chimera spider silk gene is introduced to the fistula in the presence of the helper plasmid to the inside in order to provide the stable transformant the mutation silk-worm is transformed. Therefore, these transgenic silkworms provide the creature which is suitable for for commercial production of the silk fiber producing efficiently, the spider silk.

Blessings to All

TRUTH