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Thursday, 12/28/2017 12:32:57 PM

Thursday, December 28, 2017 12:32:57 PM

Exosomal proteins as potential markers of tumor diagnosis

Aichun Li, Tianbao Zhang, Min Zheng, Yanning LiuEmail author and Zhi ChenEmail author
Journal of Hematology & Oncology201710:175
Published: 27 December 2017

Immunocapture assays

Immunocapture assays can isolate specific subpopulations of exosomes using beads, plate-coated monoclonal antibodies target specific ligand expressed on exosomal surface such as proteins or phosphatidylserine (PS) [47, 48]. Commercial kits like Magcapture™ Exosome isolation kit PS and CD63 Dynabeads® beads are based on this principle [49]. Immunocapture assays have the advantages of being high throughput, rapid (4–12 h), easy to use, and compatible with routine bench equipment and therefore are clinically applicable. Recently, the level of exosomal PS in circulation was confirmed as a hopeful marker for early diagnosis of malignancies by using sensitive ELISA [50].

https://jhoonline.biomedcentral.com/articles/10.1186/s13045-017-0542-8