Avid Bioservices Inc (CDMO)

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Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative

Figure 4 Comparison of total lipid content of (A) U937 cells, (B) CDNs and (C) exosomes derived from 2?×?107 U937 cells. (D) Lipid comparison of 4 major classes in U937 cells, CDNs and exosomes. Lipids: Sphingomyelin (SM), Phosphatidylserine ...

However, we noted that the ratio of phosphatidylcholine (PC) to the other lipids in CDNs was much higher than that in exosomes and in the parent U937 cells. This difference in lipid ratios may allude to the different properties of each vesicle type (Fig. 4D). Interestingly, the high phosphatidylcholine profile of CDNs shares some resemblance to erythrocytes, where 65% to 75% of phosphatidylcholine resides in the exterior36. Furthermore, a lower percentage of phosphatidylserine (PS) (0.3%) was observed in CDNs as compared to exosomes (2%) and U937 cells (5%). Overexpression of phosphatidylserine is postulated to increase clearance by the Mononuclear Phagocyte System (MPS), thereby reducing circulation time in vivo 36. Taken together, the reduction in phosphatidylserine and the similarity to erythrocytes may further aid in improving cellular uptake and in vivo circulation time.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662560/

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