Micro Imaging Technology Inc (MMTC)

[besidestillwater]

The easy takeaways i get from the two new thesis i saw are that NMU isnt leaving out trying to

develop 'novel' proceedures based on 'gold standard' methods for their research studies.

But i doubt if they stop there..

and the main reasons are that their tests with the 'MIT-1000A' system for 'Staph' revealed a

100% accuracy rate and those are confirmed by marquette general hospital who was part of
the actual blind study!!!!

a second reason is that by using and even trying to improve the 'gold standard methods'
with RT-PCR following actual CDC GUIDELINES they were only able to
to obtain 93% percent accuracy finding 'STAPH' for all their best efforts.

PERSONNALY, I THINK THEY ARE ON TO SOMETHING BIG AT NORTHERN MICHIGAN UNIVERSITY...

NOONE CAN SET ASIDE WHAT THEY JUST PROVED!!!!!!
(AND DUKE HAS SEVERAL COLLABORATIONS IN PLACE, READY TO EXPAND ANYTIME THEY SAY)

LET'S WATCH THE MARKET REACTION TO $MMTC !!!!!!!

AND LETS SEE WHAT HAPPENS NEXT AT NMU AND IN DR SHARP'S LAB WITH THE MIT-1000A !!!!!!!!

BECAUSE THE MIT1000 ROCKS !!!!!!!!!!!!!

here are the 2 thesis' i have found so far, more may be posted as the semester is coming to

a close soon......

1
MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND

IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS

Melissa J. Pierpont, Northern Michigan University

Date of Award

12-2016



Degree Type

Thesis



Degree Name

Master of Science



Department

Clinical Sciences



First Advisor/Chairperson

Dr. Paul Mann



Second Advisor

Catherine Bammert



Third Advisor

Dr. Josh Sharp



Fourth Advisor

Dr. Robert Winn



Abstract

Staphylococcus aureus and Streptococcus pneumoniae are bacteria that commonly colonize

healthy individuals without causing disease. Methicillin-resistant S. aureus (MRSA), a more

virulent type of S. aureus, is carried by a small percentage of people. These two bacteria

have an adversarial relationship both in vitro and in vivo, with S. pneumoniae being able to

limit the growth of S. aureus. It has been hypothesized that the relationship between these

bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine,

raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the

carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may

provide important information to support or refute this hypothesis. To facilitate this

study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to

simultaneously detect S. aureus and S. pneumoniae in the same sample.
...


http://commons.nmu.edu/bio_theses/

In this study we sought to develop a multiplexed real time PCR assay to
simultaneously detect S. aureus, MRSA, and S. pneumoniae in nasal samples. While
other studies have assessed carriage rates of these bacteria, none previously used nucleic
acid amplification based assays, but rather used conventional culture based methods
(Regev-Yochay et al, 2004.) It is widely accepted that PCR based methods have an
improved level of sensitivity when compared to culture and thus, can detect lower levels
of bacteria. As such it’s possible these studies under estimated the actual carriage rates.


At the time this project was initiated no commercially available nucleic acid
amplification test existed for this purpose. As such, we initiated a project to develop a
novel assay designed to detect low level carriage of these organisms in the context of S.
pneumoniae vaccination. The original intent was to develop a TaqMan probe based assay
as this method provides for a high level of specificity based on the combination of unique
primer and probe sequences.

........////





Recommended Citation

Pierpont, Melissa J., "MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE

SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS

AUREUS" (2016). All NMU Master's Theses. 116.





...........................
...........................
...........................
2
Polly Hockberger, Northern Michigan University
Date of Award

12-2016



Degree Type

Thesis



Degree Name

Master of Science



Department

Clinical Sciences



Program

Training and Human Performance Improvement



First Advisor/Chairperson

Paul Mann



Second Advisor

Josh Sharp



Third Advisor

Cathy Bammert



http://commons.nmu.edu/theses/117/
.........................

Evaluation of a Multiplex Real-Time PCR Assay to Simultaneously Detect Streptococcus

pneumoniae and Staphylococcus aureus in Nasal Samples

..................

No S. pneumoniae was detected in the in any of the S. aureus positive nasal samples that
were spiked with S. pneumoniae.



...........
(see references)

Pierpont, M. J. (2016). Multiplex real time PCR and melt curve assay development for
the simultaneous detection and identification of Streptococcus pneumoniae and
Staphylococcus aureus (Unpublished master's thesis). Northern Michigan
University, Marquette, MI.


.....

Appendix A: Institutional Review Board Approval Notice
Memorandum
TO: Paul Mann, Cathy Bammert, Josh Sharp, Yuba Gautam Clinical Lab Science, Biology
DATE: August 13, 2015
FROM: Brian Cherry, Ph.D. Assistant Provost/IRB Administrator
SUBJECT:IRB Proposal HS15-678 IRB Approval Dates: 8/13/2015- 8/13/2016** Proposed Project

Dates: 8/1/2015-8/1/2016 "Relationship between methicillin-resistant Staphylococcus aureus

nasal colonization and vaccination with a pneumococcal vaccine in adults"
The Institutional Review Board (IRB) has reviewed your proposal and has given it final

approval. To maintain permission from the Federal government to use human subjects in

research, certain reporting processes are required.
A. You must include the statement "Approved by IRB: Project # HS15-678" on all research

materials you distribute, as well as on any correspondence concerning this project.



http://commons.nmu.edu/do/search/?q=JOSH

%20SHARP........... ;)