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Re: natvik post# 748

Tuesday, 01/17/2017 8:15:46 PM

Tuesday, January 17, 2017 8:15:46 PM

Post# of 1060
So here too is why the longer reads look to make sense to me;

"so
the partners teamed up to better
understand the genes associated with
this resistance. They tried short-read
sequencing, but after eight library
preparations and investing over a
year on the project, they were still
missing 16 percent of the genome,
and the assembly contained highly
fragmented genes. Deeper sequence
analysis suggested the assembly,
in addition to containing gaps, had
problems with repeat regions and
large structural variations."

So the researchers switched to the Pac Bio machines;

"They had
worked on the short-read assembly
for a year, but it took just a couple
of library preparations and a single
month to get data far superior to
the original assembly. Additionally,
complex regions such as repeats and
structural variants were represented
cleanly in the PacBio assembly,
avoiding problems seen in the shortread
assembly."
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