The Zhabilov patents disclose a method for isolating and preparing an inactivated pepsin fraction (IPF) useful for detecting and treating cancers. One of the major goals of immune therapy is to harness a patient's immune system against tumor cells. The technology developed over some ten years by Zhabilov, involves treatment to increase the immunogenicity of cancer cells. This immunogenicity leads to an induction of anticancer immune response, more especially, cancer specific T cells responses. IPF increases levels of interference (IFNs). Clear demonstrations are available of increased immunogenicity of treated melanoma cells. Immunogenicity of the vaccine is further enhanced through the inclusion of proprietary adjuvant IL2. This adjuvant acts to protect the immune cells activated in response to the vaccine. This protection increases survival and prolongs the post vaccine immune response.
With regard to cancer therapy the objective is to direct the patient's immune system against tumor cells by targeting antigens that are associated with tumor cells, but not normal counterparts. These tumor associated antigens (TAAs) have been difficult to identify. Certain tumor cells express antigens that are normally not expressed, or expressed at very low levels in the healthy cells. One example is an alpha-fetoprotein, which is expressed by liver cancer cells. Another onco fetal tumor antigen is carcinoembrionic antigen (CII) which is expressed in adenocarcinomas of the digestive system. Unfortunately the immune system cannot recognize specific tumor antigens and reject the tumor. Recent advances of our understanding have revealed that any proteins binding with specific tumor antigens can be recognized by the immune system. Attached proteins by the tumor antigens form super antigens which will increase production of antibody against the tumor cells. Amplification of immune response will be using different kinds of cytokines.
A complex of inactivated pepsin fraction (IPF) component and gp96 peptide is a method of modulating immune system activity by inducing specific T cells (CTL) response against tumor cells. Gp96 also delivers maturation signals to the DC, and includes the expression of MHC antigen. The ability of gp96 to transfer antigen peptides/MHC to initiate T cell mediated anti-tumor responses and uptake and processing of tumor antigens by DC, makes it an ideal candidate for triggering an immune responses in an organism in response to tumor. It could be assumed that gp96 is binding by the immune system and this new super antigen stimulates immune responses using a non conventional antigen processing pathway. IPF is a sterile biological product. It is isolated pepsin enzyme by buffer extraction and cleavage. The following have been demonstrated in several experiment studies:
Increased CD8 and, IFN gamma
Increased CD8 number over time
Improved cellular response of both CD4 and CD8 when activated in vitro by nonspecific mitogens.
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