Huge news today on ETST. Doing research on Human Brain Cells !!! with a big biotech firm in cali .....
DV Biologics, a Leading Biological Company, Reports Earth Science Tech (ETST) High Grade Hemp CBD (Cannabidiol) Oil Reduces Oxidative Stress and Lipid Peroxidation on Human Brain Cells In Vitro
RATON, FL--(Marketwired - June 23, 2015) - Earth Science Tech, Inc. (ETST) ("ETST" or "the Company"), a unique biotechnology company focused on nutraceuticals, bioceuticals and dietary supplements, announced today that DV Biologics, a leading biological company, has confirmed ETST High Grade Hemp CBD (Cannabidiol) Oil decreased oxidative stress in human brain cells in vitro. The scientific study which was done independently by DV Biologics, a company that provides biological tools and research for the advancement of medicine, illustrates that using ETST High Grade Hemp CBD (Cannabidiol) Oil can rescue the detrimental effects of hydrogen peroxide on human brain cells (see study below). Interestingly, all the varying concentrations of ETST High Grade Hemp CBD (Cannabidiol) Oil used in this study worked similarly to rescue the detrimental effects of hydrogen peroxide on human brain cells. This data suggest that ETST High Grade Hemp CBD (Cannabidiol) Oil may have neuroprotective properties. This is the first part of the independent scientific studies that will be completed DV Biologics in order to demonstrate that ETST is focused on providing the best quality and most pure High Grade Hemp CBD (Cannabidiol) Oil. The goal of these studies is to test if ETST High Grade Hemp CBD (Cannabidiol) Oil can assist as a neuroprotectant which may help with neurodegenerative disorders.
"Keeping the public informed on the progression of studies being done on ETST High Grade Hemp CBD (Cannabidiol) Oil and products is of utmost importance to us and of course to you, the public," stated Matthew Cohen, CEO of ETST. "We were informed by DV Biologics that a portion of the studies looking at the role of ETST High Grade Hemp CBD (Cannabidiol) Oil in free radical and oxidative stress formation on brain cells was completed. We decided to release this part of the data prior to completing the remainder of the studies as this data is exciting and very encouraging for us and the users of our ETST High Grade Hemp CBD (Cannabidiol) Oil. We will continue to keep the market and consumers worldwide updated and we will continue to focus on demonstrating that ETST is dedicated to providing products with scientific backing. We hope that ETST High Grade Hemp CBD (Cannabidiol) Oil may be beneficial as a dietary supplement to consumers with neurodegenerative disorders."
Dr. Gonzalez, Vice President of Research & Development for DV Biologics states, "the data is encouraging and clearly demonstrates that ETST High Grade Hemp CBD (Cannabidiol) Oil reduces lipid peroxidation in human brain cells. There are other pathways in free radical formation and oxidative stress that occurs in the brain in disorders such as Alzheimer's but lipid peroxidation is one of the key damagers of brain cells. By the request of ETST, we are looking at other pathways to support the data presented below. We should have the studies completed by mid-third quarter."
The cells used by DV Biologics in these ETST studies are of human origin and are cells that have not been genetically modified. They are called primary cells which provide alternatives to using animal cells and performing animal studies which may not translate well into the human condition. These cells allow for the specific targeted studies such as those described below which will focus on reducing free radical formation and oxidative stress in the brain.
Does Earth Science Tech (ETST) High Grade Hemp CBD (Cannabidiol) Oil have a Neuroprotective Effect? The DV Biologics Report and data below is encouraging and clearly demonstrates that ETST High Grade Hemp CBD (Cannabidiol) Oil reduces lipid peroxidation in human brain cells.
The Brain: When fat (lipids) from food is digested, it is later broken down into fatty acid molecules. The brain then uses these for raw materials to assemble the special types of lipids it incorporates into its cell membranes. Two-thirds of the brain is composed of lipids. The vast majority of the different cell types in the brain require lipids for correct transmission of signals, providing energy and structural support to our cell membranes.
Lipid Peroxidation: Cells in the body and particularly in the brain are under constant attack by free radicals and oxidative stress. There are protective mechanisms that help deter this normal damage that occurs; however, with aging the protective mechanism declines substantially and degeneration of the brain occurs.
Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA).
In this experiment, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a fluorometric complex, proportional to the amount of MDA present, after induction of peroxidation with hydrogen peroxide.
Experimental procedure:
Two 24-well plates were coated with Matrigel for 1 hour at 27° Celsius.
The plates were seeded with human neural cells at a density of 2 X 104 cells/cm2. The cells were grown in neural media supplemented with 20 ng/ml of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF).
Media was changed every 2-3 days.
CBD oil/Neural-Growth media was prepared by diluting the CBD oil in dimethyl sulfoxide (DMSO). The suspension was filtered with a 0.22 micron filter. The CBD suspension was then diluted in N-GRO media to contain the following concentrations of CBD: 10 µM, 5 µM, 2.5 µM, and 1 µM CBD.
After the cells reached 80-90% confluency, base media was replaced with media containing CBD or control media; the controls were media only, media with DMSO 1:100, and media with hydrogen peroxide (H2O2) (H2O2 was added during induction phase).
The cells were incubated under experimental conditions for 48 hours in media containing CBD.
After 48 hours, lipid peroxidation was induced by addition of H2O2 at concentration of 100 µM to certain wells in duplicates. H2O2 was added according to the following protocol:
Well ID A B C D E F
1 Media only (control) 1 µM CBD + H2O2 5 µM CBD + H2O2
2 Media +DMSO (control) Media+2.5µM CBD only Media+10µM CBD only
3 Media + H2O2 2.5 µM CBD + H2O2 10 µM CBD + H2O2
4 Media+1µM CBD only Media+5µM CBD only
The cells were incubated with H2O2for 120 minutes at 37° Celsius.
Media was aspirated and the cells were lysed.
Total protein was precipitated, and reaction to form MDA-TBA adduct was performed. The reactions were placed in a 96-well plate.
MDA fluorometric detection was done by measuring fluorescence intensity (excitation 532 nm/ emission 553 nm) of samples and standard curve.
Relative lipid peroxidation was measured by subtracting blank value from all readings.
Results
Figure legend: Earth Science Technology (ETST) High Grade Hemp CBD (Cannabidiol) Oil is protective against lipid peroxidation in human brain cells in vitro. Human brain cells were incubated with media (control), hydrogen peroxide (H2O2) to cause oxidative stress, DMSO which is the solvent to dilute CBD in media and different concentrations of CBD with and without H2O2. Error bar = Standard deviation.
When brain cells are incubated with H2O2 by itself lipid peroxidation occurs substantially as seen in the graph above. Lipid peroxidation as measured my MDA present is seen to reach greater than 2.5 nM as compared to controls of media and DMSO alone which register 0.5 nM or less. When human brain cells are treated with ETST High Grade Hemp CBD (Cannabidiol) Oil in concentrations varying from 1 µM to 10 µM prior to the addition of H2O2 (prophylactically), human brain cells are protected against the damaging effects of lipid peroxidation.
Summary
Lipid peroxidation is damaging to human brain cells. ETST High Grade Hemp CBD (Cannabidiol) Oil in varying concentrations rescues the damaging effects of lipid peroxidation to human brain cells. The data suggest that ETST High Grade Hemp CBD (Cannabidiol) Oil may serve as a neuroprotectant to brain cells. Further studies on other oxidative stress and free radical pathways are needed in order to support and extend this data.
FOOD AND DRUG ADMINISTRATION (FDA) DISCLOSURE: These statements and products have not been evaluated by the FDA and are not intended to diagnose, treat or cure any disease. Always check with your physician before starting a new dietary supplement program.
ETST invites all interested parties to contact the company at info@earthsciencetech.com in regards to securing its "High Grade Hemp CBD (Cannabidiol) Oil" in bulk for wholesale, resale, private labeling or distribution. CBD (Cannabidiol) is a natural constituent of hemp oil and legal to consume, sell and possess in the U.S.A. and over 40 countries as dietary supplement. From product formulating, mixing, labeling and launching ETST can do it all. ETST is always open to forming new strategic partnerships, joint ventures and/or supplying vendors, resellers and third party distribution companies with its High Grade Hemp CBD (Cannabidiol) Oil/Products at wholesale prices in order to better help supply consumers as well as the health and wellness markets on a large scale.
