Pacific Biosciences of California Inc (PACB)

[Paulieme]

Thursday, April 9, 2015
Advances in Genome Assembly With Long Reads-
This symposium is co-hosted by the National Science Foundation and the
University of Minnesota Supercomputing Institute for Advanced Computational Research. MSI wordmark
Support from PacBio is gratefully acknowledged. PacBio logo


Genome Assembly is in the midst of a paradigm shift due to the plummeting cost of long-read sequencing and other large-scale physical mapping technologies. Specifically, single Molecule Real-Time (SMRT) sequencing by Pacific Biosciences (PacBio), a third-generation sequencing technology, is quickly supplanting Illumina and other second-generation sequencing technologies for whole genome assembly applications. For less than $700 in sequencing costs at Mayo, microbial genomes can be sequenced and assembled into a single contiguous high-quality genome by this technology in more than 90% of cases; whereas nearly any depth of Illumina sequencing will result in a fragmented genome consisting of hundreds of pieces. As costs continue to decline, and raw sequences become longer, complete sequence assembly is already becoming economical for fungi and simple plants. Personalized human genomes are not far off. Concurrent with these developments, Oxford Nanopore’s minion sequencer is moving beyond protypical status to offer long reads on a sequencer as small as a flash drive, and physical mapping technology companies such as BioNano and DoveTail genomics are enabling researchers to build ever bigger physical superscaffolds with confidence.

In this symposium, we present perspectives from some of the world’s leading computational scientists in the field who are experimenting with these new technologies toward the goal of creating bigger, better and more contiguous genome sequences for use in real biological contexts.

To register for the symposium, including lunch, please fill in the Symposium Registration Form.

To submit a poster to the Poster Session, please fill in the Poster Submission Form.

Please note that there is a separate registration for the optional lecture/hands-on tutorial in the morning. To attend this tutorial, please fill out the tutorial sign-up page. Space is limited. Due to high demand, two additional hands-on tutorials have been added Friday, April 10 and Tuesday, April 21.



Schedule



9:00 - 11:30: Lecture and hands-on tutorial on PacBio SMRT Portal and Assembly; Jon Badalamenti, Bond Lab [NOTE: Separate registration is required for this tutorial; please visit the tutorial sign-up page.]

11:30 - 12:30: Lunch

12:30 - 12:40: Welcome

12:45 - 1:45: Michael Schatz, CSHL: Oxford Nanopore and PacBio assembly approaches

1:45 - 2:45: Poster session

2:45 - 3:30: Jason Miller, JCVI: PacBio / Hybrid assembly

3:30 - 3:50: Peng Zhou, NSF-funded graduate student: Medicago project, University of Minnesota

3:50 - 4:30: Joann Mudge, NCGR: PacBio and BioNano

4:30 - 4:50: Wrap-up / discussion
https://www.msi.umn.edu/events/genome-assembly-mini-symposium