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Thursday, May 04, 2006 10:55:40 PM
Actually, you can't "score" a genotype until the SNP's have been discovered. And since they didn't exist in the public databases, only resequencing would produce the data needed to identify the SNP. It's a chicken and egg thing...I wouldn't expect a frog to know.
You see tadpole, the problem back in 2000 and 2001 was that the human genome sequence that DID exist came from only a few individuals, and the vast majority of it came from only a single individual. There was only about 10% overlap that came from multiple individuals due to overlapping clones of different chromosomes that were sequenced. And since a SNP must have a frequency of at least 1% in coding regions, and 10% in non-coding regions to even QUALIFY as a SNP...well, I'm sure you can see the problem for the SNPsters.
Since these SNP's didn't exist in the public database, and since there is a frequency threshold required to even qualify as a SNP, the only way to "score" those 500 individuals was to first have "completely sequenced" the relevant genes and identified the SNP's. Ergo, ipso, facto, you're on the wrong side of this argument...AGAIN.
Later,
W2P
P.S. You REALLY need to get a better understanding of the terms "sequencing" and "re-sequencing" before our next exchange. This is getting embarrassing.
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