Pacific Biosciences of California Inc (PACB)

[Paulieme]

Nanopore sequencing using charge blockade labels
(US Patent #8652779 B2) Publication number US8652779 B2
Publication type Grant
Application number US 13/893,891
Publication date Feb 18, 2014
Filing date May 14, 2013
Priority date Apr 9, 2010
Also published as US20130240359
Publication number 13893891, 893891, US 8652779 B2, US 8652779B2, US-B2-8652779, US8652779 B2, US8652779B2
Inventors Stephen Turner, Jeffrey Wegener
Original Assignee Pacific Biosciences Of California, Inc.
Export Citation BiBTeX, EndNote, RefMan
Patent Citations (31), Non-Patent Citations (54), Referenced by (1),
Abstract
The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.
Images(24)
(US20100331194 * Apr 9, 2010 Dec 30, 2010 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods)! http://www.google.com/patents/US8652779