Pacific Biosciences of California Inc (PACB)

[Paulieme]

September 11, 2014
Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
Chemistry/Chemical Biology, Northeastern University, 110 Forsyth Street, Boston, Massachusetts 02115, United States
§ Pacific Biosciences, 1380 Willow Road, Menlo Park, California 94025, United States
Nano Lett., Article ASAP
DOI: 10.1021/nl503134x
Publication Date (Web): September 11, 2014
Copyright © 2014 American Chemical Society
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ACS AuthorChoice + 12, Open Access on 09/11/2015
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.

Keywords: SMRT-sequencing; DNA sequencing; single molecule; nanophotonics; zeptoliter--- http://pubs.acs.org/doi/abs/10.1021/nl503134x?journalCode=nalefd