Pacific Biosciences of California Inc (PACB)

[Paulieme]

Journal of Microbiological Methods
Available online 27 June 2014

Improved performance of the PacBio SMRT technology for 16S rDNA sequencing
Jennifer J. Moshera, 1, Brett Bowmanb, Erin L. Bernbergc, Olga Shevchenkoc, Jinjun Kana, Jonas Korlachb, Louis A. Kaplana, -------Abstract
Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of > 1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples.

Keywords
PacBio; SMRT sequencing; 16S
Corresponding author at: Stroud Water Research Center, 970 Spencer Rd., Avondale, PA 19311, United States. Tel.: + 1 610 268 2153x228; fax: + 1 610 268 0490.
1Current address: Marshall University, Department of Biological Sciences, Huntington, WV, United States
http://www.sciencedirect.com/science/article/pii/S0167701214001675