Pacific Biosciences of California Inc (PACB)

[Paulieme]

Applications of PacBio Single Molecule, Real-Time (SMRT) DNA Sequencing
Apr 25th 12:00 pm - 1:00 pm
Room 3320, Faculty of Pharmaceutical Sciences - 2405 Wesbrook Mall, Vancouver, BC


Details:
Genomic data have become commonplace in most branches of the biological sciences and have fundamentally altered the way research is conducted.

However, the predominance of high-throughput, short-read sequence data from second-generation technologies has commonly resulted in fragmented and partial genomic and transcriptomic data, limiting the type of genetic variation able to be characterized. Long, unbiased reads from SMRT sequencing now allow for a return to more contiguous and comprehensive views of genomes and transcriptomes.

I will present several examples highlighting this transition, including improved de novo genome assemblies, characterizing structural genetic diversity, and resolving previously unsequenceable genomic regions. In the area of RNA-seq, I will highlight numerous examples of full-length transcript recall and splice isoform characterization in human and other complex transcriptomes that demonstrate that the identification of transcript isoforms and even gene content is far from complete.

Speaker Bio:

Luke Hickey is Director of Marketing and Business Development at Pacific Biosciences. He has 15 years of experience in the genome technology industry, holding various R&D, Marketing and Commercial roles at Pacific Biosciences, Affymetrix, Ingenuity Systems, and Incyte Genomics.

He currently leads development of applied markets for PacBio’s single molecule long read sequencing technology. In this role, he has contributed to co-developing novel methods and applications of the technology resulting in numerous high impact publications, including; Sequencing of the Fragile X gene, full length transcript isoform sequencing, compound somatic mutation phasing, gene editing outcome profiling, long read de novo assembly methods (HGAP/Quiver), human structural variation sequencing, centromere sequencing, haplotype phasing, and methylation profiling for epigenetic analysis.

He is on the steering committee for the 100k Pathogen project, an active participant in the NIST Genome in a Bottle Initiative, the 1,000 Genome Project, and a member of Toastmasters International. He is based in San Francisco, California with his wife and three children.

Date and Location:
Date: Friday, April 25th, 2014.
Time: 12pm - 1pm
Location: Room 3320, Faculty of Pharmaceutical Sciences. 2405 Wesbrook Mall, Vancouver, BC.

http://www.genomebc.ca/news-events/event/applications-pacbio-single-molecule-real-time-smrt-dna-sequencing/?eID=28