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"You are such a pertinent person and powerful influencer of IPIX that the evil forces somehow manipulated the system to label you as bearish"
Although I have never viewed myself in that manner who am I to question your judgement?
As you can see. I already did. It
was free, opensource
"Has anyone popped the $26 or so bucks for a copy of the article?"
Why do that when you can download it from the linked page?
Too funny. Must be something nefarious. You are such a pertinent person and powerful influencer of IPIX that the evil forces somehow manipulated the system to label you as bearish.
Bullshit. Never happened.
No need to guess:
"Brilacidin (N4, N6-bis(3-(5-gaunidinopentanamido)-2-(R)-pyrrolidin-3-yl)oxy)-5-(trifluoromethyl)phenyl)pyrimidine-4,6-dicarboxamide tetrahydrochloride) (C40H50F6N14O6.4HCl), MW 1082.7, sterile and >98% pure, was supplied by Innovation Pharmaceuticals, Wakefield, MA, USA. It was supplied as a solid and was readily soluble in water and liquid media, such as RPMI-1640. To convert µg/mL, as expressed in this paper, to millimolar, multiply µg/mL by 0.924. "
Would they call that "funding"?
Lol. You can start and end with the CEO profiles. Comparing Jensen Huang with the idiot Ehrlich is like comparing gold to a turd. Nvidia almost went bankrupt in its first two years of operation but it actually had a smart group with real employees who persevered and succeeded. This pile of garbage has been around for years, has seen a steady decline, having reached a point of no return, has had nothing but failures, no employees and as previously mentioned, an idiot for a CEO.
IPIX is insolvent. If you’re holding the bag, your investment is gone.
"My guess is IPIX supplied the Brilacidin"
Good point. Makes sense.
*NOTE: I do not understand how my original post came to have the "Bearish" sentiment. I have never used this particular icon. It was not there when I reviewed my post after submission yesterday. This morning it was. Whether my error, a hiccup, or something nefarious I plan to be more diligent.
Business history corroborates your focus on IPIX's science.
As you noted:
'60 Minutes', in their April 28, 2024 segment, Nvidia's CEO noted that the company almost went bankrupt in 1997.
Today, Nvidia is one of four companies with a greater than $2 Trillion market capitalization.
Here's the story.
https://www.cbs.com/shows/video/D3imFunju2h3IVpXugUKbxjEhVTMc1Gg/
Thanks for posting this.
The key point to note is that is usual with in vitro brilacidin tests an inoculum was used.
That is to say brilacidin was already present when the potential pathogen was introduced.
The implication of this is that if brilacidin were to be employed clinically it would have to be administered prophylactically, that is before exposure to the pathogen had occurred.
It’s probably a tad impractical to administer IV brilacidin requiring an in-patient procedure to a healthy population as a precautionary measure, especially as well over 70% of those receiving it would experience either hypertensive spikes or peripheral neuropathy. Or both.
On the other hand brilacidin is useful to add to the publication list of post-grads eager to curry favor with their supervisor.
Has anyone popped the $26 or so bucks for a copy of the article? I may, but will call a huge local library first, to see if it is on the shelves. But probably not. I almost hate to see this as it opens a glimmer of hope on a stock I had rally mostly thought of as a complete loss. Of course some hope is better than none. I just pulled it as I was bouncing around.
Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule
by David J. Larwood 1,2,3ORCID andDavid A. Stevens 2,4,*ORCID
1
Department of Pharmaceutical Chemistry, University of California-San Francisco, San Francisco, CA 94158, USA
2
California Institute for Medical Research, San Jose, CA 95128, USA
3
Valley Fever Solutions, Tucson, AZ 85719, USA
4
Division of Infectious Diseases and Geographic Medicine, Stanford University Medical School, Stanford, CA 94305, USA
*
Author to whom correspondence should be addressed.
Antibiotics 2024, 13(5), 405; https://doi.org/10.3390/antibiotics13050405 (registering DOI)
Submission received: 8 February 2024 / Revised: 16 April 2024 / Accepted: 17 April 2024 / Published: 28 April 2024
Down load key board_arrow_down Versions Notes
Abstract
Natural host defensins, also sometimes termed antimicrobial peptides, are evolutionarily conserved. They have been studied as antimicrobials, but some pharmaceutical properties, undesirable for clinical use, have led to the development of synthetic molecules with constructed peptide arrangements and/or peptides not found in nature. The leading development currently is synthetic small-molecule nonpeptide mimetics, whose physical properties capture the characteristics of the natural molecules and share their biological attributes. We studied brilacidin, an arylamide of this type, for its activity in vitro against fungi (40 clinical isolates, 20 species) that the World Health Organization has highlighted as problem human pathogens. We found antifungal activity at low concentrations for many pathogens, which indicates that further screening for activity, particularly in vivo, is justified to evaluate this compound, and other mimetics, as attractive leads for the development of effective antifungal agents.
Keywords: brilacidin; synthetic nonpeptide mimetics; antifungal activity; defensins; antimicrobial peptides; AMP
1. Introduction
Peptide antibiotics (e.g., vancomycin, daptomycin, polymyxin, echinocandins) have shown their value in clinical medicine. There are >2000 discovered natural “antimicrobial peptides” (AMPs) which are highly evolutionarily conserved and present in microbes, plants, and all vertebrates [1,2]; >100 are known to be produced by humans [3,4]. A broad antimicrobial spectrum is a group characteristic, most are amphiphilic and cationic [3]. These peptides are better termed “host defense peptides” or defensins, because they are part of the host’s innate immune response and are the first line of defense [5,6]. Many of these appear to have broad biological functions, as will be further discussed.
There has been longstanding interest in exploiting such molecules, and their analogues, as clinical anti-infectives, with stimulation to expand our armamentarium owing to the development of resistance to current chemically synthesized molecules and other natural products. Natural AMPs may be undesirable for clinical therapeutics because of instability, degradation by host proteases, low solubility, reduced activity in the presence of salts or DNA, short half-lives in vivo, difficult and expensive manufacturing issues, and the possibility of the development of antibodies in heterologous hosts [6,7,8,9,10,11]. This led to the development of synthetic AMPs, using amino acid sequences and/or amino acids not found in nature, which ameliorated some of these problems [12,13]. It was then discovered that the physicochemical properties of the synthetic molecules were more important than the sequence of the amino acids [10,11,14], and, with attention to the secondary structure, charge, and folding, that totally synthetic non-peptide molecules could recapitulate the structural properties of AMPs and mimic their activities [10,11]. A lead candidate from this line of research is brilacidin, a cationic water-soluble amphiphilic helical arylamide with discrete nonpolar hydrophobic and polar hydrophilic regions and a polymer backbone [10]. The present study is an initial exploration of the antifungal spectrum of brilacidin, with particular attention to pathogens for which there is a huge present clinical burden (e.g., cryptococcosis in Africa in the wake of the AIDS epidemic) and those pathogens for which present clinically available antifungals provide insufficient efficacy.
2. Results
The screening of the selected fungal pathogens of great interest is displayed in Table 1. The low MIC values (largely < 4 µg/mL) of all in this group, except A. fumigatus, suggests that brilacidin is worthy of study in animal models to ascertain whether this level of potency in vitro will translate into efficacy in vivo and thus have potential clinical utility. These MIC values in µg/mL are favorable compared to those of conventional antifungals.
Table 1. Brilacidin activity against problem pathogens.
There is a disparity between this 50% inhibition and the elevated 100% inhibition MICs for Coccidioides, Mucorales, Sporothrix, and Fusarium, suggesting that for those pathogens, brilacidin’s antimicrobial activity is unlike that of polyenes. Polyenes, such as amphotericin B, typically have similar concentrations for 50% and 100% inhibition and even for cidal activity [15]. However, the clinical utility of azoles and echinocandins, which also do not produce even 100% fungal inhibition in vitro, suggests that conclusions about the efficacy of brilacidin in vivo need to be deferred until animal models are explored. The most striking, consistent results are those against C. neoformans, where brilacidin appears to have unique antifungal activity among these pathogens assayed.
The studies displayed in Table 2 represent an initial screening effort to examine whether other groups of pathogens may be worthy of the broader screening displayed in Table 1. Several of these pathogens are in the favorable range discussed for pathogens studied as per Table 1 and should be more extensively screened in the future; the initial results with Nakaseomyces glabratus and Candida auris do not as yet, unfortunately, give such indication.
Table 2. Initial screen of Brilacidin activity against other problem pathogens.
3. Materials and Methods
3.1. Drugs
Brilacidin (N4, N6-bis(3-(5-gaunidinopentanamido)-2-(R)-pyrrolidin-3-yl)oxy)-5-(trifluoromethyl)phenyl)pyrimidine-4,6-dicarboxamide tetrahydrochloride) (C40H50F6N14O6.4HCl), MW 1082.7, sterile and >98% pure, was supplied by Innovation Pharmaceuticals, Wakefield, MA, USA. It was supplied as a solid and was readily soluble in water and liquid media, such as RPMI-1640. To convert µg/mL, as expressed in this paper, to millimolar, multiply µg/mL by 0.924.
In prior studies for some isolates, as mentioned, azoles were supplied by Pfizer Inc., Groton, CT, USA; echinocandins by Merck, Inc., Rahway, NJ, USA; and amphotericin B by the Bristol-Myers Squibb Company, Princeton, NJ, USA.
3.2. Isolates
The World Health Organization has recently identified particular fungal pathogens as needing attention because of epidemiological reasons and/or resistance to many available drugs [16]. It was this document that guided our selection of isolates, constrained by the availability of isolates in our collections. The isolates were all recent clinical isolates, sent to our laboratories for clinical testing, with three exceptions (CN9759, Silv., 10AF), which were originally clinical isolates but were maintained in the laboratory because they have desirable characteristics for animal model studies, which may be indicated in the future. All were tested using their CIMR accession numbers, without any patient identification.
3.3. Testing
Testing was performed by standard broth dilution methods detailed elsewhere [17,18,19]. The RPMI-1640 medium is desirable because it is fully defined and it also allows microbial susceptibility testing in the presence of mammalian cells in the future. Testing of Coccidioides was performed under BSL3 conditions. A stock solution was made of 640 µg/mL. The range of concentrations tested, in 2-fold dilutions, was 0.5–64 µg/mL. For the testing of a new drug, it is not clear whether a 50% inhibition endpoint for yeasts (equivalent to a Minimum Effective Concentration, that concentration producing a morphological change in filamentous fungi), as is used clinically for azoles and flucytosine, or a 100% inhibition endpoint (i.e., a tube as clear as the starting inoculum), as is used clinically for polyenes, is most relevant, so both endpoints were determined for brilacidin. In isolated instances where relevant (mentioned in the tables) azole resistance was defined as 50% inhibition at ≥64 µg/mL, echinocandin resistance as 50% inhibition at ≥3.1 µg/mL, and amphotericin intermediate as 100% inhibition at ≥2 µg/mL. Testing was repeated in approximately 20% of the assays and was always reproducible. Every assay included a positive concurrent control, embodying a pan-susceptible Candida kefyr, and fluconazole (MIC < 0.5 µg/mL).
4. Discussion
The activities of AMPs have been described against bacteria, protozoa, and viruses [2,20,21,22]. Several theoretical models exist to explain their interactions with cells [2,22,23]. The antifungal activity of other AMPs and their analogues has previously been demonstrated [3,4,12,13,24,25,26,27,28], including, in our prior study, against pathogens resistant to specific antifungals [13] and with cidal activity sometimes demonstrated [13,27]. A topically applied AMP has already shown antifungal efficacy in patients [23]. In the present study, conidia or yeasts were used as the inoculum. The conidia develop during the assay to hyphae; thus, in the case of filamentous organisms, antifungal activity against conidia themselves, during transformation to hyphae or on hyphal development, could produce positive test results. Prior studies have indicated AMP activity against all these phases [4,29]. Our results, with our testing methods, are consistent with the observed rapid antifungal action of AMPs [13,24]. The present study shows brilacidin activity in vitro against several problem fungal pathogens. For possible clinical interest, these studies must be expanded to further study brilacidin’s pharmacology, tissue penetration, and toxicology. What is not yet understood is why there are the species differences in susceptibility that we have demonstrated, and this may relate to differences in susceptibility to the mechanism(s) of drug action. More studies, with other fungal species, are required. Although brilacidin has been shown to depolarize the A. fumigatus cell membrane and to disrupt the cell wall [30], our results (minimal activity against this genus) present a difference from the inhibitory activity against A. fumigatus demonstrated for some AMPs [4]. A caution regarding this subject is that some AMPs have also been shown to increase A. fumigatus growth in vitro [4,27].
Prior studies have indicated the synergy in vitro of AMPs and their analogues with conventional antimicrobials and antifungals [7,8,26,28,30], even with host AMPs [8], which is an avenue for further exploration. One possible mechanism for any such synergy is that AMP increases the permeability of, and depolarization of, the pathogen membrane, allowing greater penetration of the conventional drug [6,31,32]. Brilacidin synergy with an antifungal in vivo has been shown [30].
It is unclear what in vitro test characteristics, aside from whether to use 50% or 100% endpoints, will be most useful to predict activity in vivo. Which medium is the best needs determination, as well as the conditions of pH, ionic concentration, oxygenation, and buffer [29]. It may be most relevant to study these agents in the presence of host cells, and, depending on the target in vivo, to test in a milieu that reflects the tissue situation, such as artificial sputum medium, as we have done [33]. Testing against fungal biofilms may be more relevant than against planktonic growth for many clinical situations [34], and AMPs have been demonstrated to inhibit biofilms [1,8,13,26,35].
Mechanisms of action for AMPs and their analogues include: insertion into pathogen (and host) membranes (with creation of pores) or other phospholipids and/or into ribosomal subunits, stress on protein folding, stress of cell membranes, increase of reactive oxygen species; affecting intracellular calcium concentrations, affecting the proteome, inactivation of cellular proteins; affecting cell signaling, the regulation of cell death, binding the anionic nucleic acids and/or affecting their synthesis, preventing biofilm formation, regulating iron metabolism, the inhibition of cellular enzymes, the activation of cell wall lytic enzymes, binding of glucan and/or chitin, the modulation of the cell wall to expose beta glucan, and the degradation of cell walls [1,2,3,4,6,7,8,12,23,25,27,28,36,37].
Given AMP’s effects on the regulation of many genes in their targets [6] and all these possible mechanisms of action, many effects on host function have also been described for them, including affecting host cell differentiation, immunomodulation, the regulation of cytokines, opsonization, the regulation of inflammation, the increase of phagocytosis, the stimulation of chemotaxis (for neutrophils, monocytes, and lymphocytes), the activation of eosinophils and angiogenesis, and the activation of epithelial cells [1,2,3,7,27,38]. It is likely that these possible host effects would come into play if brilacidin were to be used as an antifungal in vivo, and this may make MIC’s absolute values, or differences, in vitro less important for the effect on the outcome.
The development of resistance to AMPs has been shown generally difficult for microbes to achieve [6], and that has been corroborated for peptide AMPs [4], synthetic peptides [13], and brilacidin [10]. AMP action on several different microbial processes, as detailed above, may explain AMP’s breadth of microbial spectrum [3], as shown in our results here with various species, and AMP’s defense against resistance development [1]. Previous observations of the development of resistance to AMPs have included the development of microbial efflux pumps, which may be lessened for the nonpeptide mimetics [8]. The cationic nature of brilacidin and its water solubility may relate to its ability to target charged fungal membranes [2,11]. Brilacidin depolarization of microbial membranes and its induction of membrane and cell wall stress have been demonstrated [10].
The structure of the nonpeptide mimetics preserves the AMP theme of such biologically active molecules having both a charged face and a hydrophobic face [6,39]. The activity of these mimetics is more closely linked to their physicochemical properties than the details of the structures [40]. This nature of this class of molecules allows for studies of molecular modifications that could improve efficacy and decrease undesirable effects [12]. Its manipulation of charge, amphiphilicity, hydrophobic–hydrophilic balance, and folding properties create possibilities for the future. Presently, brilacidin is being studied in human clinical trials for other indications and is not yet focused on fungal infections.
Author Contributions
D.J.L. contributed substantially to all aspects of this project including conception, funding, execution, draft writing, and review. D.A.S. contributed substantially to all aspects of this project including conception, funding, execution, draft writing, and review. All authors have read and agreed to the published version of the manuscript.
Funding
This study was supported by funds from the University of California, San Francisco, Valley Fever Solutions, the Valley Fever Americas Foundation, the Foundation for Research in Infectious Diseases, the California Institute for Medical Research, the David and Mary Larwood Family Charitable Fund, and Innovation Pharmaceuticals.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Tables of the original raw data are available from the corresponding author.
Acknowledgments
We thank William F. DeGrado, University of California, San Francisco, for his contributions to the development of the field of nonpeptide defensin mimetics, his interest in the initiation of these studies, and his critique of the manuscript.
Conflicts of Interest
David J. Larwood is employed by Valley Fever Solutions and is a PhD candidate at the University of California, San Francisco. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
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Larwood, D.J.; Stevens, D.A. Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule. Antibiotics 2024, 13, 405. https://doi.org/10.3390/antibiotics13050405
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Larwood DJ, Stevens DA. Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule. Antibiotics. 2024; 13(5):405. https://doi.org/10.3390/antibiotics13050405
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Larwood, David J., and David A. Stevens. 2024. "Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule" Antibiotics 13, no. 5: 405. https://doi.org/10.3390/antibiotics13050405
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My guess is IPIX supplied the Brilacidin
Excellent
We thank William F. DeGrado, University of California, San Francisco, for his contributions to the development of the field of nonpeptide defensin mimetics, his interest in the initiation of these studies, and his critique of the manuscript.
This study was supported by funds from the University of California, San Francisco, Valley Fever Solutions, the Valley Fever Americas Foundation, the Foundation for Research in Infectious Diseases, the California Institute for Medical Research, the David and Mary Larwood Family Charitable Fund, and Innovation Pharmaceuticals.
Thank you for posting this. I’m a little perplexed by Innovation having provided funds. Perhaps it was quite some time ago?
Funding
“This study was supported by funds from the University of California, San Francisco, Valley Fever Solutions, the Valley Fever Americas Foundation, the Foundation for Research in Infectious Diseases, the California Institute for Medical Research, the David and Mary Larwood Family Charitable Fund, and Innovation Pharmaceuticals.“
New peer-reviewed Brilacidin publication: "Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule".
I've blocked Sneery Owl becuase he never ever posts about IPIX but only sneers at other posters.
If that ever changes, please could somebody let me know?
Even idiots, trolls and pimps deserve a second chance, I always think.
One only had to look at the sticky posts to see who was controlling the narrative. It wasn’t unusual to see 80% mod posts with the remaining 20% submitted by the mod aligned.
I regarded the degree to which they’d tipped their hand with a smidgeon of begrudged amusement.
Well towards the end of her posting she was saying some pretty harsh things about Leo and the company, which I don’t blame her for, and most I agree with! Maybe that played a role in getting her banned
Not true, there is more to it....I have corresponded with her!!!!!
She became totally fed up with Ipix and Leo! I believe she just said screw it and stopped posting! Nothing more! At least on the stocktwits forum
I heard you also got her removed from stocktwits!!! Why is that???
You're wrong about this. Learn the difference between IHub administrators and Board moderators....it's significant.
She had her freedom of speech taken away!!!!!!
Wonder where Nasty Running Girl and that dude that used the stars for his prediction are? They sure disappeared faster than a fart in a wind storm when it was evident that Leo the Lip was conning everyone. Probably onto another OTC pinkie spinning their BS. Classic shorts.
MackG Alot of proposals, but no marriages.
I did the following, using 3M shares @$.002, before I said that Schwab might be able to help...I had no reason to mislead you:
https://investorshub.advfn.com/boards/read_msg.aspx?message_id=174252754
Obviously I cancelled the trade before it executed. One issue you might run into is their policy regarding the taking of a NEW IPIX position with them. I have 100 shares in one of my accounts so the 3M order would not have been a new position. Different brokers handle Expert Market orders differently and I've heard that some won't take a NEW position.
You might want to call before applying for a new account in case they aren't taking new opening orders.
This information is incorrect. I use Charles Schwab and have not been able to buy IPIX since April 17.
Thank you for this Schwab information. If it is true and I can buy I will make a new account. My accounts today are in Fidelity.
Place your order with Schwab. They'll take it. No idea whether your $.002 limit offer will get filled though.
It's just my opinion, of course, but anyone who would buy shares in a Company with a CEO who has dropped out of sight isn't being very smart.
"I own Brilacidin and some BeaMed."
At this point you can't be sure of that (https://www.sec.gov/cgi-bin/browse-edgar?company=innovation+pharmaceuticals) and even if you could there's no hard evidence to show that they are worth owning. Even before this latest slip into the abyss of the Expert Market investors as a group couldn't move this thing past two cents.
I'm not obsessed with Leo but his actions and failures to act of late have been hard to ignore.
"Company leaders can be replaced.
Any person in a company can be replace."
Idle chatter.
How would you suggest that that could be done exactly?
When new research information arrives on Brilacidin often it is a preprint. Your reflex always is to demonstrate the new information is not peer reviewed.
You want to say: maybe it is not good.
Do you think this new information on Brilacidin will change a lot when it is peer reviewed? Do you think it is false?
Do you have some example of Brilacidin preprints that changed a lot when it was peer reviewed or was false?
Liar. IPIX doesn’t own them either.
You own worthless shares of an insolvent company. That’s reality.
You focus and obsess on Mr. Ehrlich.
I do not own Mr. Ehrlich.
I own Brilacidin and some BeaMed.
Company leaders can be replaced.
Any person in a company can be replace.
Good science can not.
Brilacidin is good science.
Every month more independent evidence.
"If Brilacidin was good some one would buy it...." So many many times the same.
Pfft.
R**n needed 18 years to find some one to buy a drug. Their first one failed.
But sure it is strange I can not find a way yet to buy 3 million more Ipix for USD$6000.
"This article is a preprint and has not been certified by peer review"
But let's say that it was. Who's gonna run the trial?
"We propose BRI as a new antifungal agent against cryptococcosis." CMC, You're right. I forgot to add the best part!!
I like your favorite part of the abstract.
But I LOVE my favorite part, which is the last line of the abstract.
Message in reply to:
Thanks for sharing that information that contradicts the often repeated falsehood that the company is "dead." My favorite part is "Brilicidin alone was shown to inhibit the growth of C. neoformans, acting as a fungicidal drug at concentrations of 2.5 µM, but surprisingly also potentiated the activity of CAS against this species. We have demonstrated that BRI is potentially an important antifungal agent against cryptococcosis, particularly important for synergizing with CAS, which is otherwise ineffective against this pathogen."
https://www.biorxiv.org/content/10.1101/2024.04.10.588976v1.full
"My advice is to block the moderators and don’t waste your time reading their infantile lies."
I blocked them long ago.
Thanks for sharing that information that contradicts the often repeated falsehood that the company is "dead." My favorite part is "Brilicidin alone was shown to inhibit the growth of C. neoformans, acting as a fungicidal drug at concentrations of 2.5 µM, but surprisingly also potentiated the activity of CAS against this species. We have demonstrated that BRI is potentially an important antifungal agent against cryptococcosis, particularly important for synergizing with CAS, which is otherwise ineffective against this pathogen."
https://www.biorxiv.org/content/10.1101/2024.04.10.588976v1.full
"Back in the day I accused you of beating and beating and beating the Dead horse."
But I was right about the horse. And now you're admitting that it was dead.
"Maybe the Sec/finra will investigate you and your Cabal."
Nonsense. The truth is a very strong defense and we're all entitled to our opinions. Even you.
Here's the truth as of 4:00PM Eastern yesterday:
IPIX
Innovation Pharmaceuticals Inc (CE) (IPIX) Quote
0.003
(note: the CE designation is a copy and paste from https://ih.advfn.com/stock-market/USOTC/innovation-pharmaceuticals-ce-IPIX/stock-price )
You can lay the blame for THAT truth at the doorstep of those who have been wrong for the last dozen years or so.
http://www.ipharminc.com/senior-management
http://www.ipharminc.com/board-of-directors
Don't blame me.
Good morning, 007. How is that IPIX portfolio looking? Just to clarify your logic, if all the realists disappeared, then suddenly you would concede that this company is in a failed state with no hope?
So why isn't Ehrlich, the CEO of this mess, blowing the Brilacidin horn in the same manner? IPIX is like a ship without a captain or crew. Million dollar negative judgements, no cash, no board, no communication, no press releases in almost a year, no filings, not even on the Pink Sheets anymore, having fallen into this Expert quagmire, a cobwebbed website so full of lies and misinformation that it should be removed. Ehrlich tried to get partners for B to no avail. Alfasigma abandoned its efforts with B. If its so great, why are there no takers? Big pharma, even little pharma, certainly know more than you. You keep posting this type of info as the company descends into darkness and the price hits a 52 week low. Your IPIX portfolio must be a disaster. Misery does not need company.
Same questions:
Where's Leo?
"The Brazil team is busy."
"The Israel team remain in schedule to ask USA FDA early June for approvals to sell their new system."
You make things sound good. Seems an odd time for a CEO to disappear without a trace, doesn't it?
What will it take for you to stop dispensing hope where none is warranted?
“ And today for the first time in over a year you saw fit to comment.”
🗑️
As long as Loan Ranger, Sunny, Scotty and Zandant are here, there is still some hope here....otherwise they would be long gone!!!!!
I apologize if i left anyone out!!!!
A interesting list of international scientists who do the new Brilacidin Cryptococcus research: https://www.biorxiv.org/content/10.1101/2024.04.10.588976v1.article-info
Also interesting where they get the money: "We thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) grant numbers 2021/04977-5 (GHG) and 2022/08796-8 (CD), 2021/10599-3 (The Antimicrobial Resistance Institute of São Paulo, The Aries Project) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant numbers 301058/2019-9, 404735/2018-5, and 405934/2022-0 (The National Institute of Science and Technology INCT Funvir) (GHG), both from Brazil, the National Institutes of Health/National Institute of Allergy and Infectious Diseases from the USA, grants R01AI153356 to GHG, R01AI053721 to JWK, and R21AI178330 to TLD, JSPS KAKENHI numbers 17H06411 (YY and CB) and 23H04882 (YY) from Japan. This work was funded by the Joint Canada-Israel Health Research Program, jointly supported by the Azrieli Foundation, Canada’s International Development Research Centre, Canadian Institutes of Health Research, and the Israel Science Foundation (GHG)."
"Invasive fungal infections have a high mortality rate causing more deaths annually than tuberculosis or malaria. The global incidence of cryptococcal meningitis is estimated at more than 400,000 new cases annually, with 181,100 annual deaths. Despite the significant impact of these infections, treatment options for cryptococcosis remain limited. Cryptococcal cells are intrinsically resistant to echinocandins and they employ an arsenal of mechanisms that enable resistance to azoles, such as upregulation of the azole target gene ERG11.Taking into consideration the increased number of individuals susceptible to cryptococcal infection, the search for new antifungal agents has become more relevant than ever. Here, we present brilacidin (BRI) as a potential antifungal agent against C. neoformans. BRI is a small molecule host defense peptide mimetic that has previously exhibited broad-spectrum immunomodulatory/anti-inflammatory activity against bacteria and viruses. Recently, we showed that BRI potentiates the effect of azoles and CAS against several fungal species, decreasing Aspergillus fumigatus fungal burden in a murine chemotherapeutic model of invasive pulmonary aspergillosis and ablating disease development in a murine model of fungal keratitis. BRI alone was shown to inhibit the growth of C. neoformans, acting as a fungicidal drug at concentrations of 2.5 µM, but surprisingly also potentiated the activity of CAS against this species. We have demonstrated that BRI is potentially an important antifungal agent against cryptococcosis, particularly important for synergizing with CAS, which is otherwise ineffective against this pathogen."
https://www.biorxiv.org/content/10.1101/2024.04.10.588976v1.full
So your mission now is to F with posters who come out of the wood work.
Just to F ing repeat the same old BS that everyone is aware of.
Back in the day I accused you of beating and beating and beating the Dead horse. And here you still are, repeating the obvious...
I think someone called you Cap'n Obvious! Lol
I honestly believe you're worried about something. Why don't you comment on the amount of negative posts on a company that Retail traders can't even buy. Maybe the Sec/finra will investigate you and your Cabal.
"Today for the first time in well over a year I got some useful data from this website."
Not from IPIX you didn't....a lack of current information explains their $.00X share price and their appearance on the inaptly called "Expert Market".
And today for the first time in over a year you saw fit to comment. Did you not see the passing on of Kevetrin and the IPIX cash balance as useful data worthy of comment?
Buy more shares. So hard to resist at these prices.
Definitely not in the wise old owl category.
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Moderators zandant Minnesinger sunspotter snayeman loanranger frenchbroad |
Innovation Pharmaceuticals Inc. (IPIX)
$IPIX NEWS - http://ipharminc.com/press-release
SEC Filings https://www.sec.gov/cgi-bin/browse-edgar?company=Innovation+Pharmaceutical&owner=exclude&action=getcompany
From the most recent 10-K:
There are doubts about our ability to continue as a going concern.
We have generated revenue of $0 and $18,000 for the fiscal years ended June 30, 2023 and 2022, respectively and have an accumulated deficit of $125.3 million through June 30, 2023. These factors raise substantial doubt about our ability to continue as a going concern.
We need to raise substantial additional capital in the future to fund our operations and we may be unable to raise such funds when needed and on acceptable terms, which could prevent us from fully implementing our business, operating and development plans.
We currently have an approximate $1.0 million cash balance as of the date of this filing, but that is insufficient to complete the development and commercialization of any of our proposed products. We expect to incur costs of approximately $1.8 million in the upcoming fiscal year ending June 30, 2024 to operate our business in accordance with our business plans and budgets.
We have limited experience in drug and formulation development, the conduct of clinical trials, and may not be able to successfully develop any drugs.
Development of pharmaceutical products is a risky and time-consuming process subject to a number of factors, many of which are outside of our control. We are subject to regulatory authority permissions and approvals, most importantly the FDA. Our drug candidate(s)/indications are at early and mid-stages of development. Consequently, we can provide no assurance of the successful and timely development of new drugs, and the failure to do so could cause us to cease operations.
We may elect to deregister our common stock under the Exchange Act and suspend our reporting obligations. Such deregistration and suspension would result in less disclosure about us and may negatively affect the liquidity and trading prices of our common stock.
http://www.ipharminc.com/
About Innovation Pharmaceuticals Inc.:
Established in 2007, and headquartered in Wakefield, Massachusetts, Innovation Pharmaceuticals Inc. (formerly Cellceutix Corporation) is a publicly traded biopharmaceutical company dedicated to discovering and advancing innovative medical therapies with dermatology, oncology, anti-inflammatory and antibiotic applications.
Through ongoing research and drug development efforts, Innovation is focused on improving the clinical care of patients, as well as growing shareholder value. Our team consists of a diverse and accomplished group of professionals working together toward achieving this common goal.
IPIX CEO - "The Company historically devoted most of its efforts and resources on business development, regulatory matters, and clinical trials. Presently, the Company does not have sufficient financial resources to advance our drug candidates meaningfully." "In general, we expect to concentrate on product development and engage in a limited way in product discovery, avoiding the significant investment of time and financial resources that is generally required for a promising compound to be identified and brought into clinical trials." Source - https://www.sec.gov/ix?doc=/Archives/edgar/data/1355250/000147793223003414/ipix_10q.htm
More information is available at http://www.ipharminc.com.
Management: http://www.ipharminc.com/senior-management/
Scientific Advisors: http://www.ipharminc.com/scientific-advisors/
Clinical Trials: https://clinicaltrials.gov/ct2/results?cond=&term=%22Innovation+Pharmaceuticals%2C+Inc.%22++&cntry=&state=&city=&dist=&Search=Search&flds=abcefgps
Trials under previous name: https://clinicaltrials.gov/ct2/results?cond=&term=cellceutix&cntry=&state=&city=&dist=&Search=Search&flds=abcefgps
Product Pipeline: http://www.ipharminc.com/therapeutic-areas/
Recent News and Press Releases:
https://finance.yahoo.com/quote/IPIX?p=IPIX
http://www.ipharminc.com/press-release/
SEC Filings (CIK:0001355250): https://www.sec.gov/cgi-bin/browse-edgar?company=Innovation+Pharmaceutical&owner=exclude&action=getcompany
Transfer Agent:
West Coast Stock Transfer, Inc.
721 N. Vulcan Ave. Ste. 205
Encinitas, CA 92024
(619) 664-4780 p
(760) 452-4423 f
cs@wcsti.com
http://www.westcoaststocktransfer.com
Share Structure: See SEC filing link above for current share structure
Investor Relations:
http://www.ipharminc.com/new-faq/
http://www.ipharminc.com/contact-us-1
Chart: https://stockcharts.com/h-sc/ui?s=IPIX
All messages, including iBox content, are the opinion of the posters, are no substitute for your own research, and should not be relied upon for stock trading or any other purpose.
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