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Re: None

Monday, 12/15/2014 10:10:50 AM

Monday, December 15, 2014 10:10:50 AM

Post# of 16430
I made a breakdown of all the different EL plates listed for sale on the website. On many of them I have added to the description of the plates as provided by Nanologix to better describe their uses or limitations. I am hoping this will be useful to some who see it.


Tryptic Soy Agar
Application: For the isolation and cultivation of a variety of fastidious and non-fastidious microorganisms. TSA is also used for total plate counts and for maintaining stock cultures.

Red Blood Agar
Application: For the isolation and cultivation of a variety of fastidious microorganisms, to detect hemolytic activity, and to perform CAMP tests (identification of Group B ß-streptococci).

Nematode Growth Media
Application: For use in cultivating Nematodes. In particular Caenorhabditis elegans.
Currently an international consortium of laboratories are collaborating on a project to sequence the entire 100,000,000 bases of DNA of the C. elegans genome.

Chocolate Agar
Application: For the isolation and cultivation of fastidious microorganisms, primarily Neisseria (e.g. N. meningitidis and N. gonorrhoeae) species from clinical samples.

LB Lennox Agar
Application: For the growth of recombinant Escherichia coli, and for culturing enteric bacteria and facultative organisms.

LB Miller Agar
Application: For the growth of recombinant Escherichia coli, and for culturing enteric bacteria and facultative organisms.

LB Miller Agar(LBM0.1X) (Less Tryptone, Yeast and NaCl than the one above)
Application: For the growth of recombinant Escherichia coli, and for culturing enteric bacteria and facultative organisms.

Note: What is the difference between LB Miller and LB Lennox broths?
The LB Miller Broth and LB Lennox Broth contain higher and lower sodium chloride levels respectively. There are minimal differences between the two formulations in most molecular biology studies with commonly used E.coli strains. Different bacteria strains may require different salt concentrations. The low salt formula is more often used in salt-sensitive antibiotic selections. The LB broth used in most labs is LB Miller broth which contains 10 grams NaCl per liter of medium.

Middlebrook 7H10 Agar
Application: For the isolation and cultivation of Mycobacterium species, predominately for Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Tryptic Soy Agar-Kanamycin (TSAKAN20)
Application: For the isolation of various strains that are Kanamycin resistant, or for strains that contain plasmids that may confer Kanamycin (a common antibiotic) resistance. Kanamycin has been used to treat infections caused by Gram-negative bacteria including E. coli, Proteus spp., Serratia marcescens, and Klebsiella pneumoniae.

Tryptic Soy Agar-Kanamycin Plates (TSAKAN50) (Larger quantity of Kanamycin than TSAKAN20)
Application: For the isolation of various strains that are Kanamycin resistant, or for strains that contain plasmids that may confer Kanamycin resistance.

Tryptic Soy Agar-Carbenicillin Plates (TSACARB50)
Application: Carbenicillin (antibiotic) is an analog of Ampicillin (a form of penicillin). For the isolation of various strains that are Ampicillin resistant, or for strains that contain plasmids that may confer Ampicillin resistance. Can be used for detection of Pseudomonas aeruginosa, a sometimes fatal bacterial infection in animals (including humans).

Tryptic Soy Agar-Carbenicillin Plates (TSACARB100) (same as TSACARB50 with more Carbenicillin)

TSA-Kanamycin-Ampicillin Plates
Application: For use with ampicillin and kanamycin resistant strains and strains harboring plasmids. Similar use to those listed previously.

Tryptic Soy Agar-Ampicillin Plates
Application: For the cultivation of Escherichia coli and various antibiotic resistant bacteria. It is also sometimes used for the treatment of urinary tract infections, meningitis, and salmonella infections, but resistance to ampicillin is increasingly common among the bacteria responsible for these infections.

Tryptic Soy Agar-BioNanoPore
Application: For the detection of a variety of fastidious and non-fastidious microorganisms. Can be used with aerobic or anaerobic microorganisms. In the BNP form!

Polymyxin-Lysozyme-EDTA-Thallous acetate
Application: PLET Agar is the best selective medium for the isolation and cultivation of Bacillus anthracis (anthrax) from environmental samples, animal products, and clinical specimens.

MacConkey Agar
Application: For the selective and differential isolation of gram-negative bacilli by lactose fermentation. Designed to selectively isolate Gram-negative and enteric bacilli (normally found in the intestinal tract) bacteria and differentiate them for lactose fermentation.