Replies to post #94607 on Kraig Biocraft Laboratories Inc (KBLB)

[Cee-It]

it looks like the may have found a solution or work around to the issue

Obviously the scientific researchers have solved and blown by that issue (belief) a few years ago. Now it is a matter of "best fit technology" so that true potential is achieved at the most economic levels. MIT Labs and perhaps a dozen others have come into the market to help define and improve solutions, some into this for the profits and others for the scientific discoveries.

WHY? Because this solution projects to be so much cheaper than growing the silk within worms. Just as Rayon proved itself, the "goo" approach will as well and consequently the market will belong to those using that technology.

[TRUISM]

Here is an English link to the enzyme research team page where you can see a list of some of Keiji Numata's publications:

Here's a collaboration;Publication Date (Web): July 8, 2011:


Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery

Keiji Numata †, Michaela R Reagan †, Robert H Goldstein ‡, Michael Rosenblatt ‡, and David L Kaplan *†
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, United States
Department of Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111-1800, United States

Bioconjugate Chem., 2011, 22 (8), pp 1605–1610
DOI: 10.1021/bc200170u
Publication Date (Web): July 8, 2011
Copyright © 2011 American Chemical Society

David. L. Kaplan, Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts, 02155 USA. Phone: [phone]. Fax: [phone]. E-mail: [e-mail].

§ Author Present Address

Enzyme Research Team, Biomass Engineering Program, RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.





The present study demonstrates pDNA complexes of recombinant silk proteins containing poly(l-lysine) and tumor-homing peptides (THPs), which are globular and approximately 150–250 nm in diameter, show significant enhancement of target specificity to tumor cells by additions of F3 and CGKRK THPs.

We report herein the preparation and study of novel nanoscale silk-based ionic complexes containing pDNA able to home specifically to tumor cells. Particular focus was on how the THP, F3 (KDEPQRRSARLSAKPAPPKPEPKPKKAPAKK), and CGKRK, enhanced transfection specificity to tumor cells.

Genetically engineered silk proteins containing both poly(l-lysine) domains to interact with pDNA and the THP to bind to specific tumor cells for target-specific pDNA delivery were prepared using Escherichia coli, followed by in vitro and in vivo transfection experiments into MDA-MB-435 melanoma cells and highly metastatic human breast tumor MDA-MB-231 cells.

Non-tumorigenic MCF-10A breast epithelial cells were used as a control cell line for in vitro tumor-specific delivery studies.

These results demonstrate that combination of the bioengineered silk delivery systems and THP can serve as a versatile and useful new platform for nonviral gene delivery.



Blessings to All

TRUTH

[TRUISM]

At first I thought that this might be related to Spiber Inc. in some way, but looking into it, i don't think so anymore.

Don't be so quick to dismiss....
You may be onto something after all:



Japanese Visitors
Click For Link


A team of researchers from RIKEN and Spiber Inc. in Japan, led by Dr. Keiji Numata, visited Penang from 2-4 March 2015 for sampling of spiders in order to study the properties of spider silk, a functional biomaterial.

Harvesting of silk from spider and processing of samples were conducted in the Molecular Laboratory of Professor. Dr. K. Sudesh Kumar in Centre for Chemical Biology (CCB).


Blessings to All

TRUTH