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Monday, April 22, 2019 6:46:15 PM
https://www.freshpatents.com/-dt20161110ptan20160327567.php
Methods of applying proteins to surfaces to form arrays are well known. The immobilization may be covalent or noncovalent, via a linker moiety, or tethering to an immobilized moiety. These methods are well known in the field of solid phase synthesis and micro-arrays, for example as described in Beier et al., Nucleic Acids Res. 27:1970-1-977 (1999), and U.S. Pat. No. 6,101,946 to Martinsky. Examples include binding moieties for attaching proteinaceous molecules to a solid support include streptavidin or avidin/biotin linkages, carbamate linkages, ester linkages, amide, thiolester, (N)-functionalized thiourea, functionalized maleimide, amino, disulfide, amide, hydrazone linkages, and among others. Furthermore, a number of companies offer commercial services for preparing protein arrays, for example Arrayit Corporation (Sunnyvale, Calif.). Preferably, the source of the proteins is an extract of the allergen source, such as those available from GREER® Allergy Immunotherapy (Lenoir, N.C.). Preferably, the extract of the allergen source is standardized.
Aspects of the inventions described herein have been described on the website of ARRAYIT® Corporation (arrayit.com) and links thereon. All such disclosures were made by another who obtained the subject matter disclosed directly or indirectly from the inventor of the present application.
https://clinicaltrials.gov/ct2/show/NCT03514303
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