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Wednesday, 08/09/2017 12:51:13 PM

Wednesday, August 09, 2017 12:51:13 PM

Post# of 185857
BIG News out from $PSID yesterday!

PositiveID has successfully detected Ebola virus on the Firefly Dx breadboard pathogen detection system (prototype system) and published a white paper and data on PositiveID's website and ExcitePCR's website.

In a collaborated effort with assay partner GenArraytion Inc., ExcitePCR successfully detected the Ebola virus on the Firefly Dx prototype system. The Ebola virus was tested at a low number of copies via polymerase chain reaction (PCR) and the amplification curves demonstrated a cycle threshold (Ct) of 21. The automated runs successfully synthesized cDNA using a proprietary reverse transcriptase (RT) step and then completed a 40-cycle PCR to produce the results.

Rapid detection and diagnosis of the Ebola virus infection along with vaccination is imperative to quickly containing an epidemic. Ebola is an RNA virus and requires a RT step prior to PCR. Despite its potential diagnostic advantages, RT-PCR methodology (both conventional and real-time approaches) typically requires significant laboratory infrastructure, electrical power, multiple temperature-sensitive reagents, the operation and maintenance of specialized equipment, and technical expertise in molecular biology, potentially complicating deployment in resource-limited settings.

"At the height of the Ebola virus outbreak, there were multiple challenges with accurately diagnosing individuals due to the need to collect samples, take them back to a central lab, and then wait hours or even days for results," stated Lyle L. Probst, CEO and President of ExcitePCR."Firefly Dx could eliminate those challenges as a self-contained, portable lab for point-of-care/point-of-need applications, providing results in 30 minutes."

https://finance.yahoo.com/news/positiveid-successfully-detects-ebola-virus-120000812.html

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